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  • RAB0352 - Cell-Based ELISA Sampler Kit for detecting phospho-ERK1/2 (pThr202/pTyr204), phospho-JNK (pThr183/pTyr185), and phospho-p38 MAPK (pThr180/pTyr182) in cultured cell lines

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RAB0352 Sigma-Aldrich

Cell-Based ELISA Sampler Kit for detecting phospho-ERK1/2 (pThr202/pTyr204), phospho-JNK (pThr183/pTyr185), and phospho-p38 MAPK (pThr180/pTyr182) in cultured cell lines

192 assays

  •  NACRES NA.32

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Properties

Related Categories Antibodies, Antibody Kits, Cell-Based ELISAs, ELISA Kits
usage   192 assays
species reactivity   human, mouse, rat
application(s)   ELISA: suitable
  capture ELISA: suitable
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MAPK1(5594), MAPK14(1432), MAPK3(5595), MAPK8(5599)
mouse ... MAPK1(26413), MAPK14(26416), MAPK3(26417), MAPK8(26419)
rat ... MAPK1(116590), MAPK14(81649), MAPK3(50689), MAPK8(116554)

Description

General description

The Cell-Based ERK1/2 (activated) ELISA Sampler Kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of ERK1/2 (pThr202 / pTyr204), JNK (pThr183 / pTyr185) and p38 MAPK (pThr180m / pTyr182) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines.

Immunogen

ERK1/2 (pThr202 / pTyr204), JNK (pThr183 / pTyr185), p38 MAPK (pThr180 / pTyr182) synthetic phosphopeptides

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Kit component also available separately

Description

Product #

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5x Blocking Solution (Item F) SDS RABBLOCK
Fixing Solution (Item D) SDS RABFIX1
HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1) SDS RABHRP1
HRP-conjugated Anti-Mouse IgG Concentrate (Item I2) SDS RABHRP2
Uncoated Microplate SDS RABPLATE1
Quenching Solution for Cell-based ELISA Assay (Item E) SDS RABQUENCH
Stop Solution SDS RABSTOP1
TMB Substrate Reagent (Item J) SDS RABTMB1
20X Wash Buffer Concentrate A SDS RABWASH1
20X Wash Buffer Concentrate B SDS RABWASH2
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Target organs 
Respiratory system
RIDADR 
UN 3264 8 / PGIII

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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