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  • RAB0352 - Cell-Based ELISA Sampler Kit for detecting phospho-ERK1/2 (pThr202/pTyr204), phospho-JNK (pThr183/pTyr185), and phospho-p38 MAPK (pThr180/pTyr182) in cultured cell lines

RAB0352 Sigma-Aldrich

Cell-Based ELISA Sampler Kit for detecting phospho-ERK1/2 (pThr202/pTyr204), phospho-JNK (pThr183/pTyr185), and phospho-p38 MAPK (pThr180/pTyr182) in cultured cell lines

192 assays

  •  NACRES NA.32



Related Categories Antibodies, Antibody Kits, Cell-Based ELISAs, ELISA Kits
usage   192 assays
species reactivity   human, mouse, rat
application(s)   ELISA: suitable
  capture ELISA: suitable
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MAPK1(5594), MAPK14(1432), MAPK3(5595), MAPK8(5599)
mouse ... MAPK1(26413), MAPK14(26416), MAPK3(26417), MAPK8(26419)
rat ... MAPK1(116590), MAPK14(81649), MAPK3(50689), MAPK8(116554)


General description

The Cell-Based ERK1/2 (activated) ELISA Sampler Kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of ERK1/2 (pThr202 / pTyr204), JNK (pThr183 / pTyr185) and p38 MAPK (pThr180m / pTyr182) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines.


ERK1/2 (pThr202 / pTyr204), JNK (pThr183 / pTyr185), p38 MAPK (pThr180 / pTyr182) synthetic phosphopeptides


For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Kit component also available separately


Product #

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5x Blocking Solution (Item F) SDS RABBLOCK
Fixing Solution (Item D) SDS RABFIX1
HRP-conjugated Anti-Rabbit IgG Concentrate (Item I1) SDS RABHRP1
HRP-conjugated Anti-Mouse IgG Concentrate (Item I2) SDS RABHRP2
Uncoated Microplate SDS RABPLATE1
Quenching Solution for Cell-based ELISA Assay (Item E) SDS RABQUENCH
Stop Solution SDS RABSTOP1
TMB Substrate Reagent (Item J) SDS RABTMB1
20X Wash Buffer Concentrate A SDS RABWASH1
20X Wash Buffer Concentrate B SDS RABWASH2
Safety & Documentation

Safety Information

Signal word 
Target organs 
Respiratory system
UN 3264 8 / PGIII


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Protocols & Articles


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ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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