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RAB0372 Sigma-Aldrich

Human MMP-9 ELISA Kit

for serum, plasma, cell culture supernatant and urine

  •  NACRES NA.32

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Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   human
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
  capture ELISA: suitable
input   sample type plasma
sample type urine
sample type cell culture supernatant(s)
sample type serum
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-6000 pg/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... MMP9(4318)

Description

General description

The Human MMP-9 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP-9 pro and active forms in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Human MMP9

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Matrix metalloproteinase 9 (MMP9) is a gelatinase enzyme, which catalyzes the degradation of gelatin and collagen and is preferentially inhibited by tissue inhibitor of metalloproteinases 1 (TIMP-1). The MMP9 protein is a zinc-dependent protease which is responsible for degradation of extracellular matrix components. It also participates in the pathogenesis of cancers. MMP9 is associated with various disorders, such as cystic fibrosis, acute lung injury, asthma as well as respiratory syncytial virus infection both in vitro and in vivo. MMP-9 is involved in a variety of autoimmune diseases such as systemic lupus erythematosus, rheumatoid arthritis and multiple sclerosis and be regarded as a potential therapeutic target. Mutation of MMP-9 is associated with lumbar-disc herniation (LDH) and metaphyseal anadysplasia (MAD).

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component also available separately

Description

Product #

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ELISA 5X Assay/Sample Diluent Buffer E (Item E2) SDS RABELADE
ELISA Stop Solution (Item I) SDS RABSTOP3
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) SDS RABTMB3
20X Wash Buffer (Item B) SDS RABWASH4
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3264 8 / PGIII

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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