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RAB0603 Sigma-Aldrich

Human ALB / Serum albumin ELISA Kit

  •  NACRES NA.32

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Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   human
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
input   sample type serum
sample type plasma
sample type cell culture supernatant(s)
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 6 ng/mL
standard curve range: 4.915-1200 ng/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... ALB(213)

Description

General description

The ALB ELISA kit provides for the quantitative measurement of Albumin in Cell Culture Supernatants, Plasma and Serum.

This ELISA (enzyme-linked immunosorbent assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of a target protein in biological samples, such as serum, plasma, cell culture supernatants, urine and/or cell and tissue lysates.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Human serum albumin (HSA) is a single, non-glycosylated chain that binds to various endogenous and exogenous ligands. It functions as a plasma transporter molecule and primarily binds to non-esterified long-chain fatty acids. Albumin also binds to and transports various metabolites, such as bilirubin, steroid hormones, thyroxin, tryptophan, certain vitamins and metal ions within the body. It also has the ability to bind to several drugs and affects their pharmacokinetics and pharmacodynamics. HSA functions as a nitric oxide-carrier and also influences the antioxidant capacity of human serum. In cases of acute hemolysis, HSA binds to heme in the blood stream and transports to hemopexin, where in it is reabsorbed by parenchymal liver cells.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component only

Description

Product #

Add to Cart

Human ALB Antibody-coated ELISA Plate (Item A)    
Human ALB Protein Standard, Lyophilized (Item C)    
Biotinylated Human ALB Detection Antibody (Item F)    
HRP-Streptavidin (Item G)    
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)    
ELISA Stop Solution (Item I)    
20X Wash Buffer (Item B)    
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3264 8 / PGIII

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

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Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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