• USA Home
  • RAB0614 - Human APOE / Apolipoprotein E 2 ELISA Kit

EMAIL THIS PAGE TO A FRIEND
RAB0614 Sigma-Aldrich

Human APOE / Apolipoprotein E 2 ELISA Kit

  •  NACRES NA.32

Purchase

Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   human
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
input   sample type cell culture supernatant(s)
sample type serum
sample type plasma
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 61 pg/mL
standard curve range: 61.44-15000 pg/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... APOE(348)

Description

General description

The ApoE2 ELISA kit provides for the quantitative measurement of ApoE2 in cell culture supernatants, plasma and serum.
The APOE gene is mapped to human chromosome 19q13.32 and codes for a 299 amino acid protein. The APOE protein is localised to the brain and liver. In the central nervous system, APOE is mostly produced by astrocytes and neurons to a lesser extent.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

APOE is responsible for the translocation of cholesterol and phospholipids to the cells. APOE is known to be associated with neuroinflammatory and neurodegenerative diseases. APOE regulates cognitive behaviour, and hence related to neurocognitive disorders that are influenced by HIV infection or Alzheimer′s disease.

Kit component also available separately

Description

Product #

Add to Cart

ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) SDS RABTMB3
ELISA Stop Solution (Item I) SDS RABSTOP3
20X Wash Buffer (Item B) SDS RABWASH4
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3264 8 / PGIII
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?