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RAB0631 Sigma-Aldrich

Human C5a / Complement C5a ELISA Kit

for serum, plasma, and cell culture supernatants

  •  NACRES NA.32

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Properties

Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
species reactivity   human
packaging   kit of 96 wells (12 strips x 8 wells)
application(s)   ELISA: suitable
input   sample type cell culture supernatant(s)
sample type serum
sample type plasma
assay range   inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 31 pg/mL
standard curve range: 31.25-2000 pg/mL
detection method   colorimetric
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... C5(727)

Description

General description

Human complement 5a (C5a) is generated during complement activation from the α-chain of complement C5. It binds to a signaling G-protein coupled receptor (GPCR), inducing neutrophil chemotaxis and endothelial cell activation. C5a also triggers an oxidative burst in macrophages and neutrophils and induces release of histamine in basophils and mast cells.

The antibody pair provided in this kit recognizes human Complement Component C5a.

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit component also available separately

Description

Product #

Add to Cart

ELISA Colorimetric TMB Reagent (HRP Substrate, Item H) SDS RABTMB3
ELISA Stop Solution (Item I) SDS RABSTOP3
ELISA 1X Assay/Sample Diluent Buffer A (Item D1) SDS RABELADA
ELISA 5X Assay/Sample Diluent Buffer B (Item E1) SDS RABELADB
20X Wash Buffer (Item B) SDS RABWASH4
Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
RIDADR 
UN 3264 8 / PGIII
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Protocols

ELISA Protocols | Sandwich ELISA Protocol & More Types

NOTE: ALL incubations and wash steps must be performed under gentle rocking or rotation (~1‐2 cycles/sec).
Keywords: Cell culture, Phosphorylations, Size-exclusion chromatography

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Peer-Reviewed Papers
15

References

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