EMAIL THIS PAGE TO A FRIEND
RDD002 Sigma-Aldrich

HEPES

anhydrous, free-flowing, Redi-Dri, ≥99.5%

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein/REAXYS Number 883043

  •  EC Number 230-907-9

  •  MDL number MFCD00006158

  •  PubChem Substance ID 329824215

  •  NACRES NA.25

Purchase

Properties

Quality Level   100
grade   anhydrous
product line   Redi-Dri
assay   ≥99.5%
form   crystalline powder
quality   free-flowing
pH   5.0-6.5 (25 °C, 238 g/L)
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
solubility   water: 500 mg/mL, clear, colorless
SMILES string   OCCN1CCN(CC1)CCS(O)(=O)=O
InChI   1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key   JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
• In the novel vesicle-budding protocol as a component in stock solution and buffer solutions
• As a component in intracellular solution for the preparation of brain-slice recording
• As a component of wash buffer, elution buffer and desalting buffer during affinity protein purification
• To maintain the pH at 7 in thermal denaturation assay of purified protein
• In isocratic elution of protein using gel filtration
• Along with NaCl in sample preparation for EPR (Electron Paramagnetic Resonance) spectroscopy

Packaging

1 kg in poly bottle

2.5 kg in poly drum

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Buffers - From Bnech to Bulk
Protocols & Articles

Articles

Enjoy the Free-flowing Experience - Redi-Dri™

What is the most frustrating moment you have experienced in the lab? Inadequate chemicals for the reaction? Or rock-solid material as you open your bottle when you only need a small amount? If you ha...
Keywords: Environmental

Protocols

Redi-Dri™ FAQs

A time study was performed to test the Red-Dri Packaging System against standard packaging. Equivalent units of both packaging systems containing potassium chloride (KCl) were introduced to a control...
Keywords: Absorption

Related Content

Redi-Dri™ – The world of free-flowing hygroscopic products

The Redi-Dri™ packaging system eliminates the common issue of clumping that is noted with many hygroscopic salts and buffers and provides free-flowing, ready-to- use material at all times. Don’t wast...
Keywords: Buffers, Chromatin immunoprecipitation, Environmental, Reductions, Sample preparations

Redi-Dri™ – 뭉치고 덩어리지지 않는 시약의 세계

From our library of Related Content, Sigma-Aldrich presents Redi-Dri™ – 뭉치고 덩어리지지 않는 시약의 세계

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?