PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production., suitable for cell culture

Synonym: N,N-Bis(2-hydroxyethyl)glycine, BICINE

  • CAS Number 150-25-4

  • Empirical Formula (Hill Notation) C6H13NO4

  • Molecular Weight 163.17

  •  Beilstein/REAXYS Number 1769362

  •  EC Number 205-755-1

  •  MDL number MFCD00004295

  •  PubChem Substance ID 329824252

  •  NACRES NA.21



Related Categories Biochemicals and Reagents, Biological Buffers, Good’s Buffers, PharmaGrade Buffers
Quality Level   400
biological source   synthetic
Agency/Method   non-compendial
product line   PharmaGrade
form   powder
application(s)   cell culture | mammalian: suitable
impurities   Endotoxin and microbial; tested
useful pH range   7.6 - 9.0
pKa (25 °C)   8.3
suitability   suitable for manufacturing use
foreign activity   Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
InChI   1S/C6H13NO4/c8-3-1-7(2-4-9)5-6(10)11/h8-9H,1-5H2,(H,10,11)


General description

PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit


Bicine is a biological buffer often referred to as a “Good’s” buffer. The pKa of Bicine is 8.3 which makes Bicine is a good candidate for biological applications that require pH control above physiological. Bicine buffers are commonly used in electrophoresis and protein separations. Bicine is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.

Bicine can be used in a wide array of biological applications including as a component in media formulations. Specific applications include buffers for electrophoresis, protein purification and diagnostic reagent production.

Recommended buffer for low temperature biochemical work. Used in the preparation of stable substrate solution for serum guanase determination. Spacer in plasma protein fractionation with Sephadex® by isotachophoresis.


Product is available in the following package sizes:
RES1151B-B701X: 100 gm container
RES1151B-B702X: 1 kg container
RES1151B-B704X: 10 kg container


Enhanced Quality Product Documentation on PharmaGrade Bicine

Legal Information

Sephadex is a registered trademark of GE Healthcare

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

pHast Pack
Protocols & Articles

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