RES1151B-B7 Sigma


PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production., suitable for cell culture

Synonym: N,N-Bis(2-hydroxyethyl)glycine, BICINE

  • CAS Number 150-25-4

  • Empirical Formula (Hill Notation) C6H13NO4

  • Molecular Weight 163.17

  •  Beilstein Registry Number 1769362

  •  EC Number 205-755-1

  •  MDL number MFCD00004295

  •  PubChem Substance ID 329824252



Related Categories Biochemicals and Reagents, Biological Buffers, Good’s Buffers, PharmaGrade Buffers
Quality Level   ELITE
biological source   synthetic
grade   Non-Compendial
product line   PharmaGrade
form   powder
impurities   Endotoxin and microbial; tested
useful pH range   7.6 - 9.0
pKa (25 °C)   8.3
suitability   suitable for cell culture
  suitable for manufacturing use
foreign activity   Cytotoxicity, DNase, NICKase, RNase, and Protease; tested



Bicine is a biological buffer often referred to as a “Good’s” buffer. The pKa of Bicine is 8.3 which makes Bicine is a good candidate for biological applications that require pH control above physiological. Bicine buffers are commonly used in electrophoresis and protein separations. Bicine is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.

Bicine can be used in a wide array of biological applications including as a component in media formulations. Specific applications include buffers for electrophoresis, protein purification and diagnostic reagent production.

Recommended buffer for low temperature biochemical work. Used in the preparation of stable substrate solution for serum guanase determination. Spacer in plasma protein fractionation with Sephadex® by isotachophoresis.

General description

PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit


Enhanced Quality Product Documentation on PharmaGrade Bicine


Product is available in the following package sizes:
RES1151B-B701X: 100 gm container
RES1151B-B702X: 1 kg container
RES1151B-B704X: 10 kg container

Legal Information

Sephadex is a registered trademark of GE Healthcare

Safety & Documentation

High Quality GMP-Buffers

Protein Stabilizer
Protocols & Articles

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