RES3077T-A7 Sigma


PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production, suitable for cell culture

Synonym: N-[Tris(hydroxymethyl)methyl]glycine, Tricine

  • CAS Number 5704-04-1

  • Linear Formula (HOCH2)3CNHCH2CO2H

  • Molecular Weight 179.17

  •  Beilstein Registry Number 1937804

  •  EC Number 227-193-6

  •  MDL number MFCD00004277

  •  PubChem Substance ID 329824267



Related Categories Biochemicals and Reagents, Biological Buffers, Good’s Buffers, PharmaGrade Buffers
Quality Level   ELITE
biological source   synthetic
grade   Non-Compendial
product line   PharmaGrade
form   powder
impurities   Endotoxin, microbial, and trace metals; tested
useful pH range   7.4 - 8.8
pKa (25 °C)   8.1
suitability   suitable for cell culture
  suitable for manufacturing use
foreign activity   Cytotoxicity, DNase, NICKase, RNase, and Protease; tested



Buffer component for separation of low molecular weight peptides.

Tricine is a biological buffer often referred to as a “Good’s” buffer. The pKa of Tricine is 8.1 which makes Tricine is a good candidate for biological applications that require pH control above physiological. Tricine is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.

Tricine can be used in a wide array of biological applications including as a component in media formulations. Specific applications include buffers for electrophoresis, protein purification and diagnostic reagent production.

General description

PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit


Enhanced Quality Product Documentation on PharmaGrade Tricine


Product is available in the following package sizes:
RES3077T-A701X: 100 gm container
RES3077T-A702X: 1 kg container
RES3077T-A704X: 10 kg container
RES3077T-A705X: 25 kg container

Safety & Documentation

High Quality GMP-Buffers

Protein Stabilizer
Protocols & Articles

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