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RES6003H-B7 Sigma

HEPES

PharmaGrade, Manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production, suitable for cell culture

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein Registry Number 883043

  •  EC Number 230-907-9

  •  MDL number MFCD00006158

  •  PubChem Substance ID 329824273

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Good’s Buffers, PharmaGrade Buffers
Quality Level   ELITE
biological source   synthetic
grade   Non-Compendial
InChI Key   JKMHFZQWWAIEOD-UHFFFAOYSA-N
product line   PharmaGrade
form   powder
impurities   Endotoxin, microbial, and trace metals; tested
pH   4.7-5.3 (, )
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
suitability   suitable for cell culture
  suitable for manufacturing use
foreign activity   Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

Description

Application

HEPES is a biological buffer often referred to as a “Good’s” buffer. It is the most commonly used biological buffer since the pKa of HEPES is 7.31 which corresponds to the physiological pH of 7.36. HEPES is considered to be non-toxic to culture cell lines, highly water soluble and provides high-solution clarity.

HEPES is used in cell culture media, biopharmaceutical buffer formulations (both upstream and downstream) and diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.

General description

PharmaGrade products are manufactured under appropriate controls for use as a raw material in pharma or biopharmaceutical production. For more information on the PharmaGrade Program, visit sigma-aldrich.com/safc-pharmagrade.

Linkage

Enhanced Quality Product Documentation on PharmaGrade HEPES

Packaging

This product is available in the following package sizes:
RES6003H-B701X: 100g container
RES6003H-B703X: 5kg container
RES6003H-B705X: 25kg container
RES6003H-B706X: 50kg container

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TL6809000

High Quality GMP-Buffers

Protein Stabilizer
Protocols & Articles

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Peer-Reviewed Papers
15

References

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