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S0192 Sigma-Aldrich

Stemline® II Hematopoietic Stem Cell Expansion Medium

Serum-free, contains L-glutamine, liquid, sterile-filtered, suitable for cell culture

  •  NACRES NA.81



Related Categories 3D Stem Cell Culture using 3D Biotek 3D Insert Scaffolds, Cell Biology, Cell Culture, Cell Culture Supplies, Culture,
Quality Level   500
sterility   sterile-filtered
form   liquid
storage condition   protect from light
application(s)   cell culture | mammalian: suitable
shipped in   wet ice
storage temp.   2-8°C


General description

The second generation of Sigma′s hematopoietic stem cell expansion media family, Stemline II has been developed to optimize the balance of differientiated and undifferentiated cells while maximizing their expansion. Compatible with Hematopoietic Stem Cells (HSC) from bone marrow, cord blood, and mobilized peripheral blood, Stemline II has been shown to lead to significant increases in cell expansion from all three sources. Through flow cytometric analysis of clinical-scale expansions, Stemline II has also demonstrated a higher capacity than other commercially available media for the expansion of CD34+/CD38+ late progenitors required for short-term engraftment. Human cord blood cells expanded in Stemline Media demonstrate impressive self-revewal when transplanted into immunodeficient NOC/SCID mice, illustrating Stemline′s utility in true functional trial.

Stemline Hematopoietic Stem Cell Expansion Medium free of serum and all other animal-derived components with the exception of human serum albumin. This exclusion increases performance consistency and elimates safety risks associated with potential adventitious agents.

Produced in a GMP state-of-the-art facility, Stemline Hematopoietic Stem Cell Expansion Medium is clearly an excellent choice for your HSC applications.

Please click here to contact us if you are considering including the use of this product in an Investigational New Drug application.


Must be supplemented with growth factors and cytokines according to individual user protocols.

Legal Information

Stemline is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Hematopoietic Stem & Progenitor Cell Culture Protocols

Isolation of Human CD34+ Hematopoietic Progenitor Cells Expansion of Human CD34+ Hematopoietic Progenitor Cells The Colony Formation Unit (CFU) Cell Assay  
Keywords: Cell culture, Centrifugation, Clinical, Culture media, Flow cytometry, Gene expression

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