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S0266 Sigma-Aldrich

Spermidine

BioReagent, for molecular biology, suitable for cell culture, ≥98%

Synonym: 1,8-Diamino-4-azaoctane, N-(3-Aminopropyl)-1,4-diaminobutane

  • CAS Number 124-20-9

  • Linear Formula NH2(CH2)3NH(CH2)4NH2

  • Molecular Weight 145.25

  •  Beilstein/REAXYS Number 1698591

  •  EC Number 204-689-0

  •  MDL number MFCD00008229

  •  PubChem Substance ID 24899434

  •  NACRES NA.52

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Properties

Related Categories Biochemicals, Molecular Biology, Molecular Biology Reagents More...
Quality Level   200
grade   for molecular biology
product line   BioReagent
assay   ≥98%
form   solid or liquid
application(s)   cell culture | mammalian: suitable
refractive index   n20/D 1.479 (lit.)
density   0.925 g/mL at 25 °C (lit.)
foreign activity   DNase, RNase, and protease, none detected
storage temp.   room temp
SMILES string   NCCCCNCCCN
InChI   1S/C7H19N3/c8-4-1-2-6-10-7-3-5-9/h10H,1-9H2
InChI key   ATHGHQPFGPMSJY-UHFFFAOYSA-N
Gene Information   rat ... Ppm1a(24666)

Description

General description

Spermidine belongs to the natural polyamines, that are found in chloroplasts and in bacterial or eukaryotic cells.

Application

Spermidine has been used in the preparation of gold beads and as a component in nuclei isolation buffer (NIB).

Spermidine is suitable for use in molecular biology buffers and for precipitation of DNA >100 bp in length. It was used to deliver DNA in plants and mammalian cells by particle bombardment technique.

Biochem/physiol Actions

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T4 polynucleotide kinase activity.

Spermidine helps to maintain the proper activity of gene expression. This polyamine plays a key role in intermediate metabolism. It also serves as an inhibitor of the acetyl transferase activity of E1A-associated protein p300 (EP300). Spermidine participates in cell growth.

Caution

Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3259PSN2 8 / PGII
WGK Germany 
WGK 3
RTECS 
EJ7000000
Flash Point(F) 
233.6 °F - closed cup
Flash Point(C) 
112 °C - closed cup

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
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The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Is this a spermidine solution or a neat liquid, and what is the concentration?
Based on a density of 0.925 g/mL and a molecular weight of 145.25, this neat liquid has an effective molarity of 6.4 M.
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Protocols & Articles
Peer-Reviewed Papers
15

References

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