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S0692 Sigma-Aldrich

Spectinomycin

Ready Made Solution, 100 mg/mL in DMSO/H2O, 1:1

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Properties

Related Categories Aminoglycosides, Antibacterial, Antibiotic Ready Made Solutions, Antibiotics, Antibiotics A to Z,
biological source   Streptomyces sp.
form   solution
concentration   100 mg/mL in DMSO/H2O, 1:1
color   colorless
solubility   DMSO/H2O, 1:1: soluble 100 mg/mL
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
  mycobacteria
shipped in   wet ice
storage temp.   −20°C

Description

Biochem/physiol Actions

Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.

Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).

Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.

Caution

Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.

Packaging

1 mL in glass bottle

Preparation Note

Stock solutions should be prepared at 10 mg/mL in water and 0.2 μm filtered.

Application

Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.

General description

Chemical structure: aminoglycoside

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage. Store under inert gas

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
Flash Point(F) 
199.4 °F
Flash Point(C) 
93 °C

Documents

Certificate of Analysis

Protocols & Articles

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Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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