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S0948 Sigma

Anti-SIRT4 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-SIR2L4, Anti-Sirtuin (silent mating type information regulation 2 homolog) 4, Anti-sir2-like 4, Anti-sirtuin 4, Anti-sirtuin type 4

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation, Antibodies for Gene Regulation and Expression,
species reactivity   mouse (predicted), human, rat (predicted)
application(s)   indirect immunofluorescence: 2-4 μg/mL using human SH-SY5Y cells
  western blot: 2-4 μg/mL using whole extracts of HEK-293T cells expressing recombinant human SIRT4
clone   polyclonal
concentration   ~1.0 mg/mL
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt ~34:30 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... SIRT4(23409)
mouse ... Sirt4(75387)
rat ... Sirt4(304539)
biological source   rabbit
antibody product type   primary antibodies
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 302-314 of human SIRT4, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat Sirt4.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Primary and Secondary Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay needs. Use the Antibody...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Therapeutic uses, Western blot

抗体基础知识

免疫球蛋白(Ig)由B淋巴细胞产生并分泌到血浆中。单体形式的Ig分子是一个分子量约为150 kDa的糖蛋白,且其结构差不多类似字母Y。Ig单体的基本结构(图1)包括由两个二硫键连接的两个相同的半体。每个半体都具有一个约50 kDa的重链和一个约25 kDa的轻链,两者在轻链羧基末端附近通过二硫键连接在一起。重链可分为Fc部分和Fab部分,前者位于羧基末端(Y的基座),后者则位于氨基末端(Y的臂)...
Keywords: Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Rheumatology

Peer-Reviewed Papers
15

References

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