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  • S100-05N - Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC: Pre-Screened for Angiogenesis & VEGF signaling, neonatal

S100-05N Sigma-Aldrich

Human Dermal Microvascular Endothelial Cells: CADMEC/HMVEC: Pre-Screened for Angiogenesis & VEGF signaling, neonatal

  •  NACRES NA.81



Related Categories Cell Culture, Endothelial Cells, Pre-Screened, Primary Cell Culture, Primary Cells More...
Quality Level   100
biological source   human blood vessels
packaging   pkg of 500,000 cells
growth mode   Adherent
karyotype   2n = 46
morphology   Endothelial
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C


General description

Endothelial cells (EC) line the blood vessels of the entire circulatory system, from aorta to capillaries, and act as a barrier between circulating blood and the rest of the vessel wall/surrounding tissues. Endothelial cells are involved in the processes of angiogenesis, vasculogenesis, vasoregulation, coagulation, fibrinolysis and selective blood barrier. They are also implicated in pathophysiological processes, including cardiovascular disease and cancer development.

The most commonly used human endothelial cells are Human Umbilical Vein Endothelial Cells, (HUVEC), Human Microvascular Endothelial Cells (HMVEC) and Human Aortic Endothelial Cells (HAOEC). Select lots of HUVEC, HMVEC, and HAOEC have been pre-screened to demonstrate stimulation-dependent angiogenesis and key endothelial cell signaling pathways (phosphorylation of VEGFR, Akt, MAPK, and expression of Tie2, eNOS, Axl and Etk/Bmx.

VEGF-Stimulated Signaling in Pre-Screened Endothelial Cells
VEGF receptor-2 (VEGFR-2) is a major VEGF of endothelial cells. VEGFR-2-mediated signaling plays a critical role in angiogenesis, including regulation of proliferation, differentiation, cell movement, and survival of endothelial cells. VEGF-induced receptor dimerization triggers activation of VEGFR-2 tyrosine kinase and autophosphorylation at a specific set of tyrosine residues, which serve as docking sites for downstream signaling components leading to activation of downstream signaling molecules, including phosphorylation of Akt and p44/42-MAPK.

Expression of Signaling Biomarkers in Pre-Screened Endothelial Cells
Prescreened Endothelial Cells express the following important biomarkers:
• Tie2, a receptor tyrosine kinase critical for the angiogenic remodeling, sprout formation, survival of endothelial cells and vessel stabilization processes; eNOS (endothelial Nitric Oxide Synthase), the enzyme that producse NO which is an important signaling molecule that regulates a diverse range of physiological events and is required for normal endothelial function;
• Axl, a receptor tyrosine kinase whose ligand is the survival factor Gas6 (growth arrest-specific gene 6 product) implicated in many processes, such as cell survival, leukocyte transmigration and neointima formation.
• Etk/Bmx (Endothelial/epithelial Tyrosine Kinase), a member of the Btk family, participates in signal transduction stimulated by growth factor receptors, cytokine receptors, G-protein-coupled receptors, antigen receptors, and integrins, and has been implicated in cell adhesion, migration, proliferation, and survival.

Cell Line Origin

Blood Vessels


Endothelial vessel function, cell signaling, angiogenesis


Cell Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

• 2nd passage, >500,000 cells in Cell Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 16 doublings

Subculture Routine

Please refer to the CADMEC/HMVEC Culture Protocol.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

Please Enter a Lot Number

Pipette Controller from Brand
Protocols & Articles


Protocol: Human Dermal Microvascular Endothelial Cells Culture

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