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S1452 Sigma-Aldrich

MISSION® siRNA Transfection Reagent

Synonym: MISSION® siRNA Transfection Reagent

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Properties

Related Categories Cloning and Expression, Functional Genomics and RNAi, MISSION siRNA, Mission siRNA Transfection Reagent, Molecular Biology,
product line   MISSION®
form   aqueous solution
shipped in   wet ice
storage temp.   2-8°C

Description

General description

The MISSION® siRNA Transfection Reagent is for the transfection of siRNA to achieve ≥90% silencing efficiency for transient knockdown of eukaryotic gene expression.
Effective gene silencing and reduced off target effects are achieved using a low siRNA concentration (1nM). The siRNA Transfection Reagent is provided as a sterile solution, and is compatible with serum and antibiotics.

Other Notes

Gene silencing assay: An A549 cell line stably expressing firefly luciferase was transfected in 24-well plates with 8.4 ng of 21-mer Luciferase siRNA using 2 μL of the MISSION® siRNA Transfection Reagent. Experiments were performed in triplicate in presence of serum. Cell lysates were assessed for luciferase expression 48 hours post-transfection.

Legal Information

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Warning
Hazard statements 
RIDADR 
UN1170 - class 3 - PG 3 - Ethanol, solution
WGK Germany 
3
Flash Point(F) 
120.2 °F
Flash Point(C) 
49 °C

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Articles

Transfection Reagent Selection Guide

Choosing a transfection reagent is easier when you can rely on the work of researchers who have used it before you. During development of each reagent, good transfection efficiency was verified in a ...
Keywords: Infrared spectroscopy, Ion-exchange chromatography, Transfection

Related Content

How Transfection Works [VIDEO]

Transfection is the process that introduces DNA or RNA into eukaryotic cells. It is a common and essential method to study and modulate gene regulation, which allows for a better understanding of gen...
Keywords: Gene expression, Genomics, Proteomics, Transfection

Transfection Efficiency and Optimization

The two main purposes of transfection are to produce recombinant proteins and to specifically enhance or inhibit gene expression in transfected cells. As such, transfection is a powerful analytical t...
Keywords: Gene expression, Transfection

Peer-Reviewed Papers
15

References

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