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S1686 Sigma-Aldrich

Monoclonal Anti-v-Src antibody produced in mouse

clone 327, purified immunoglobulin, lyophilized powder

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphorylation Enzymes,
conjugate   unconjugated
clone   327, monoclonal
biological source   mouse
application(s)   immunoprecipitation (IP): suitable (suitable; may appear as doublet due to phosphorylation)
  western blot: 2-5 μg/mL using COLO201 cell extract
species reactivity   mouse, human, rat
mol wt   antigen ~56 kDa
form   lyophilized powder
shipped in   wet ice
storage temp.   2-8°C
antibody form   purified immunoglobulin
isotype   IgG1κ
antibody product type   primary antibodies
UniProt accession no.   P12931
Gene Information   human ... SRC(6714)
mouse ... Src(20779)
rat ... Src(83805)

Description

General description

Src family kinases are a group of structurally related non-receptor protein tyrosine kinases that can lead to a variety of cellular responses including differentiation and proliferation. All the members have an amino terminal that has the kinase function and the C-terminal that regulates the kinase function. The amino terminal has Src homology 4 domain (SH4), containing a myristylation signal sequence essential for membrane localization; conserved SH3 and SH2 domains, which mediate protein-protein interactions, separated from the SH4 by a unique region; a catalytic kinase domain. The major difference between v-Src and c-Src is the structure of the C terminal. Src is involved in the regulation of many cellular functions such as migration, adhesion, gene transcription, cell cycle, apoptosis and differentiation. Few signalling pathways through which Src regulates these processes are PI3K, STAT3, FAK and paxillin. Src overexpression is observed in human cancers.
Monoclonal Anti-v-Src reacts with active Src kinase from human, mouse, and rat detecting a band of approximately 56 kDa.

Immunogen

Src protein (p60src).

Application

The recommended anti-v-Src antibody concentration is 2-5 μg/mL for immunoblotting using a COLO201 cell extract and chemiluminescence detection. The antibody is suitable for immunoprecipitation.

Physical form

Lyophilized with 100 μg bovine serum albumin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Analysis Note

p60src may appear as a doublet due to phosphorylation.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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