• USA Home
  • S2065 - Anti-phospho-Src (pTyr529) antibody produced in rabbit

S2065 Sigma-Aldrich

Anti-phospho-Src (pTyr529) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-pp60src(pY529)



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Phosphoproteins at Phosphotyrosine (pTyr),
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
  western blot: 1:1,000 using human platelets or NIH3T3 cells
species reactivity   human, mouse, rat, chicken
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P12931
Gene Information   human ... SRC(6714)
mouse ... Src(20779)
rat ... Src(83805)


General description

Src is a proto-oncogene that regulates neuronal functions, such as nerve cell differentiation. The Src kinase family structurally comprises of SH2, SH3 and kinase domains, as well as a negative regulatory C-terminal tail. Src has also been implicated in functions such as cell growth, development and cell-cell interactions . Anti-phospho-Src (pTyr529) antibody is specific for human, mouse, rat and chicken Src (60 kDa). The product has not been tested for reactivity with Fyn and Yes (92% homologous).


The sequence is conserved in mouse, rat, chicken and frog.

synthetic phosphopeptide derived from the region of Src that contains tyrosine 529 based on the sequence of human src.


Anti-phospho-Src (pTyr529) antibody is suitable for use in western blot (1:1,000 using human platelets or NIH3T3 cells). The product may also be used for immunoprecipitation (1:1000 using HEK293 or HEL cell lysates ) and and immunohistochemistry.

Physical form

Solution in Dulbecco′s phosphate-buffered saline (without Mg2+ and Ca2+), pH 7.3, containing 50% glycerol, 1.0 mg/ml BSA (IgG and protease-free) and 0.05% sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?