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S2519 Sigma-Aldrich

Anti-Soy Protein antibody produced in rabbit

fractionated antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Miscellaneous, Plant Biology, Primary Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   dot blot: 1:500
  indirect ELISA: 1:5,000
species reactivity   soy
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   fractionated antiserum
Quality Level   200
antibody product type   primary antibodies

Description

General description

Some cross-reaction is observed with extracts from wheat flour and ovalbumin; no cross-reaction is observed with extracts from maize, rice, potato flour, casein, or meat.

Soy protein is obtained from soybeans. This legume is free of cholesterol and is low in saturated fat. It is mainly used as a replacement for animal proteins in an individual′s diet. This vegetable food has all eight essential amino acids. Soy protein can be used as meat extenders or analogues.

Immunogen

renatured soy protein extracted from soy acetone powder with hot urea.

Application

Anti-Soy Protein antibody produced in rabbit has been used in:
• enzyme-linked immunosorbent assay (ELISA)
• supershift analysis
• fluorescent imaging
• immunostaining to probe polyvinylene diflouride (PVDF)-bound proteins for cross-reactivity

Anti-Soy Protein antibody produced in rabbit was used for soy quantification in natural rubber latex glove extracts by ELISA at 1:2000 dilution. It was used as a control in supershift analysis of growth-hormone-induced protein binding to lactogenic hormone-responsive region.

Target description

Soy protein in a variety of forms is the basis of many low cholesterol foods and may also be incorporated in animal meat products.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Soy protein is a rich source of isoflavones that act as estrogen analogs. They exert both estrogenic and anti-estrogenic effects depending on the tissue in which they act. Isoflavones effect the lipid and bone metabolism and may act as a potential alternative to estrogen in post-menopausal women.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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