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  • S2562 - Anti-Stem Cell Factor Receptor (SCF R) antibody produced in goat

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S2562 Sigma-Aldrich

Anti-Stem Cell Factor Receptor (SCF R) antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-CD117

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Description

General description

Mast cells play an important role in innate immunity or the response of the body to invading pathogens. The functions of mast cells are regulated by a number of surface receptors; Stem cell factor receptor (SCF R; CD117) being the most critical. The signalling triggered by SCF R results in mast cell activation in response to antigens. The response involves tyrosine phosphorylation by specific tyrosine kinases that drive the downstream signalling process. The secondary pathways activated by SCF R include Akt, mTOR, MAPK and c-Jun. In addition to development of mast cells, SCF R also regulates their subsequent maturation and homing to the residence sites. Constitutive activation by SCF R is implicated in myeloproliferative discorders, systemic mastocytosis and gastrointestinal stomal cell tumors
Anti-human stem cell factor receptor recognizes recombinant human SCF receptors.

Immunogen

recombinant human stem cell factor receptor (SCF R) extracellular domain expressed in Sf 21 cells.

Application

Anti- stem cell factor receptor antibody may be used to neutralize human SCF R at neutralization dose (ND50) of 0.3-1.0 μg/ml. For ELISA, the recommended antibody concentration is 0.5-1.0 μg/ml. The antibody is suitable for immunoblotting at a working concentration of 0.1-0.2 μg/ml.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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