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S2647 Sigma-Aldrich

Spectinomycin dihydrochloride pentahydrate

powder, γ-irradiated

  • CAS Number 22189-32-8

  • Empirical Formula (Hill Notation) C14H24N2O7 · 2HCl · 5H2O

  • Molecular Weight 495.35

  •  EC Number 244-554-3

  •  MDL number MFCD00150886

  •  PubChem Substance ID 24899532

  •  NACRES NA.85

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Properties

Related Categories Aminoglycosides, Antibiotics, Antibiotics A to Z, Antibiotics N-S, Antibiotics by Application,
Quality Level   200
biological source   synthetic (inorganic)
grade   for molecular biology
sterility   γ-irradiated
form   powder
potency   ≥500 μg per mg
color   white to off-white
solubility   H2O: soluble at 
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
storage temp.   2-8°C
suitability   nonselective for Escherichia coli
  nonselective for coliforms
SMILES string   O.O.O.O.O.Cl.Cl.CN[C@@H]1[C@H](O)[C@H](NC)[C@H]2O[C@]3(O)[C@@H](O[C@H](C)CC3=O)O[C@@H]2[C@H]1O
InChI   1S/C14H24N2O7.2ClH.5H2O/c1-5-4-6(17)14(20)13(21-5)22-12-10(19)7(15-2)9(18)8(16-3)11(12)23-14;;;;;;;/h5,7-13,15-16,18-20H,4H2,1-3H3;2*1H;5*1H2/t5-,7-,8+,9+,10+,11-,12-,13+,14+;;;;;;;/m1......./s1
InChI key   DCHJOVNPPSBWHK-UXXUFHFZSA-N

Description

General description

Chemical structure: aminoglycoside

Biochem/physiol Actions

Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.

Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).

Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.

Packaging

100MG

Caution

Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.

Preparation Note

Stock solutions should be prepared at 10 mg/mL in water and filter sterilized.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Aminoglycoside Analysis in Pork Muscle using Molecularly Imprinted Polymer Cleanup and LC/MS/MS Detection

Aminoglycosides are a well-known class of antibiotics that are routinely monitored in animal-derived foods. Many countries have instituted regulatory limits for aminoglycosides. There is growing conc...
Emily Barrey, Olga Shimelis, K. G. Espenschied
Reporter US, Volume 32.2
Keywords: Antibiotics, High performance liquid chromatography, Mass spectrometry, Separation, Size-exclusion chromatography, Solid phase extractions

Related Content

Antibiotics Selection Guide

The Antibiotic Selection Guide enables researchers to spend more time on their research discoveries and less time seeking established antibiotic information. Link to hundreds of antibiotics for conta...
Keywords: Antibiotics, Cell biology, Cell culture, Genetic, High performance liquid chromatography, Reversed-phase chromatography

Peer-Reviewed Papers
15

References

Related Products

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Description

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S0692 Spectinomycin, Ready Made Solution, 100 mg/mL in DMSO/H2O, 1:1
S4014 Spectinomycin dihydrochloride pentahydrate, powder, BioReagent, suitable for cell culture

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