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S2937 Sigma-Aldrich

Anti-Sos1 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Primary Antibodies,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect immunofluorescence: 1:500 using using cultured canine MDCK cells.
  microarray: suitable
  western blot: 1:4,000 using whole extracts of A431 (human epidermoid carcinoma) and PC 12 (rat pheochromo­cy­to­ma) cell lines.
species reactivity   canine, rat, human
mol wt   antigen 170 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   Q07889
Gene Information   human ... SOS1(6654)
mouse ... Sos1(20662)

Description

General description

Son of Sevenless (SOS) was first discovered in Drosophila, responsible for normal eye development. Two homologs in mouse and humans have been described, Sos-1 and Sos-2; mouse Sos-1 and Sos-2 are 70% identical. Mammalian Sos proteins are present in the inner cell membrane. Sos-1 is a dual guanine nucleotide exchange factor that activates Rac1 and Ras in response to growth factors. Sos-1 can associate also with the GRAP adaptor protein and it is also capable of forming complexes that exhibit Rac-specific guanine nucleotide exchange factor activity. Along with receptor tyrosine kinases, Sos-1 plays an important role in cellular migration and proliferation. In plants, Sos-1 is important to maintain salt concentration across the cell membranes.
Anti-Sos-1 specifically recognizes Sos-1 by immunoblotting (170 kDa). An additional band of lower molecular weight may be detected in some cell line extracts.

Immunogen

synthetic peptide corresponding to the C-terminus region of SOS-1 of human origin (amino acids 1315-1333). This sequence is identical in mouse.

Application

Detection of Sos-1 by immunoblotting is possible by using a minimum antibody working dilution of 1:4,000 in whole extracts of A431 (human epidermoid carcinoma) and PC 12 (rat pheochromocytoma) cell lines. For indirect immunofluorescent staining a minimum working dilution of 1:500 may be used in cultured canine MDCK cells. The antibody is suitable for protein microarray.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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