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S3014 Sigma-Aldrich

Sodium chloride

for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)

Synonym: Halite

  • CAS Number 7647-14-5

  • Linear Formula NaCl

  • Molecular Weight 58.44

  •  Beilstein/REAXYS Number 3534976

  •  EC Number 231-598-3

  •  MDL number MFCD00003477

  •  PubChem Substance ID 24899542

  •  NACRES NA.25

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Properties

Related Categories Biochemicals, Buffer / Buffer Salts, Buffers / Buffer Salts, Buffers / Buffer salts, Buffers / Buffers Salts,
Quality Level   200
grade   for molecular biology
assay   ≥99% (titration)
form   crystalline
turbidity   ≤3.5 NTU
pH   7
mp   801 °C (lit.)
solubility   H2O: 1 g/10 mL, clear, colorless
anion traces   phosphate (PO43-): ≤5 ppm
cation traces   K: ≤50 ppm
  heavy metals (as Pb): ≤5 ppm
suitability   suitable for molecular biology
foreign activity   DNase, RNase, and protease, none detected
SMILES string   [Na+].[Cl-]
InChI   1S/ClH.Na/h1H;/q;+1/p-1
InChI key   FAPWRFPIFSIZLT-UHFFFAOYSA-M

Description

Packaging

1, 6×1 kg in poly bottle

5, 10 kg in poly drum

500 g in poly bottle

Other Notes

Easily compare specifications for Sodium Chloride products with the Sodium Chloride specification table.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
VZ4725000

pHast Pack
Protocols & Articles

Articles

HPLC Analysis of a Synthetic Oligonucleotide on TSKgel® SuperQ-5PW

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of a Synthetic Oligonucleotide on TSKgel® SuperQ-5PW
Keywords: High performance liquid chromatography

Introduction to Western Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is termed as blotting. Proteins resolved on Sodium dodecyl sulfate - polyacrylamide gel electrophor...
Keywords: Buffers, Cell disruption, Detergents, Electroblotting, Electrophoresis, Gel electrophoresis, Homogenization, Immobilization, PAGE, Protein assay, Protein extraction, Sample preparations, Separation, Size-exclusion chromatography, Western blot

Recombinant Protein Expression in Saccharomyces cerevisiae

Expressing recombinant proteins in yeast has many advantages, simple protocols, use of basic reagents, appropriate processing of eukaryotic proteins, and scalability. We are an essential part of your...
Keywords: Cell disruption, Gene expression, transformation

Protocols

Protein Extraction and Cell Lysis Protocol

All the steps for protein extraction from cells or tissue (fresh or frozen) must be carried out at 2-8 °C. The following is the composition of one common lysis buffer that is used to prepare protein ...
Keywords: BCA assay, Cell disruption, Electrophoresis, Gel electrophoresis, Homogenization, Protein assay, Protein extraction

Restriction Enzyme Cloning Manual Buffer Recipes

Tip 1: DNA is quite stable in TE buffer at 4ºC. If stored in elution buffer or water then freezing at -20 ºC is advised.
Keywords: Phase transitions, Sequencing, Sterilizations

Transforming E. coli with Engineered Plasmid

Inoue and colleagues developed this method in 1990. It works well for many strains commonly used in cloning. The original method calls for growing the overnight E. coli cultures at 18°C. We find that...
Keywords: Antibiotics, Centrifugation, Cloning, Peptide synthesis, Phase transitions, transformation

Peer-Reviewed Papers
15

References

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