• USA Home
  • S3062 - Anti-α-Synuclein antibody produced in rabbit

S3062 Sigma

Anti-α-Synuclein antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-SNCA



Related Categories Alphabetical Index, Antibodies, Antibodies for Parkinson’s Disease Research, Cell Biology, Cell Signaling and Neuroscience,
species reactivity   rat, human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using formic acid treated sections of Alzheimer′s Disease brain
  microarray: suitable
  western blot: 1:1,000 using extract of rat brain homogenate
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 19 kDa
storage temp.   −20°C
Gene Information   human ... SNCA(6622)
rat ... Snca(29219)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to a sequence near the C-terminus of human α-synuclein (amino acids 111-132 with C-terminally added lysine) conjugated to KLH. This sequence is highly conserved (90% homology) in mouse and rat α-synuclein. This sequence has no homology with β- and γ-synuclein.

General description

The synucleins are a family of soluble presynaptic proteins that are abundant in neurons and include α-synuclein, β-synuclein, and γ-synuclein. Human α-synuclein (also known as the non-amyloid component of plaques precursor protein or NACP) is a 140-amino acid polypeptide that is encoded by a gene on chromosome 4. It was originally isolated from plaques of Alzheimer’s disease (AD) brains as a 19 kDa protein precursor of the highly hydrophobic 35-amino acid peptide, nonamyloid component (NAC) of plaques. The NAC can self-aggregate into fibrils and induce aggregation of the β-amyloid peptide. α-Synuclein is highly abundant in presynaptic terminals, and is a major component of Lewy bodies (LBs). Pathogenic point mutations in the α-synuclein gene are linked to familial Parkinson’s disease (PD) in rare kindreds. However, most neurodegenerative disorders with LBs are associated with abnormal accumulation of wild-type, α-synuclein. α-Synuclein is expressed primarily in brain, but is also expressed in low levels in all tissues examined except liver.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Rabbit polyclonal anti-α-Synuclein antibody recognizes human and rat α-synuclein. Staining of α-synuclein in immunoblotting is specifically inhibited by the immunizing peptide


Rabbit polyclonal anti-α-Synuclein antibody is used to tag α-Synuclein for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques, such as immunoblotting. It is used as a probe to determine the presence and roles of α-Synuclein in plaques of Alzheimer’s disease (AD) brains.

Price and Availability

Antibody Explorer

Explore with Confidence

Celebrate Pi Day!
Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Parkinson's Disease

Parkinson's disease (PD) is the second most common neurodegenerative disorder after Alzheimer's disease, affecting more than 6 million people worldwide. PD is a slowly progressing motor system neurod...
Carolyn L. Crankshaw
BioFiles v7 n2, 2011, 16–20
Keywords: Alzheimer Disease, Apoptosis, Chromatin immunoprecipitation, Clinical, Degradations, Dopamine agents, Gene expression, Parkinson Disease, Polymorphisms


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?