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S3077 Sigma

3dGRO Spheroid Medium

Synonym: CSC media, Cancer stem cell media, tumorsphere culture media, tumorsphere media



Related Categories 3D Cell Culture Products, 3D Stem Cell Culture Products, Cell Culture Supplies, Labware
packaging   pkg of 250 mL (basal medium)


Features and Benefits

• Chemically defined, Serum-free formulation.
• High tumorsphere formation efficiency.
• Allows extended serial tumorsphere passaging (>10).
• Proven to enrich CSCs from numerous cancer cell lines.

General description

Cancer stem cell (CSCs) are defined as a small subset of cells within a tumor with the ability to self-renew and often drive tumor progression and recurrence after chemotherapy treatment. Therefore, development of specific therapies targeted at CSCs holds hope for improvement of survival and quality of life of cancer patients, especially for patients with metastatic disease.
Traditionally, cancer stem cells have been isolated from cancer cell lines and tumor biopsies and grown in undefined serum containing media using 3-dimensional cell cultures. Using these undefined culture conditions leads to experimental variability and suboptimal cell growth.
3dGRO Spheroid Medium is a new chemically defined and serum-free cancer stem cell media that supports the expansion of CSC′s in 3D spheroid cell cultures. The media supports serial passaging of cancer cell lines leading to an overall enrichment of CSC populations.

Other Notes

Cell Lines tested with 3dGRO Spheroid Medium:
MCF-7, A-431, Panc-1, LNCaP, HT-29, A-549, U-87 MG, HT1080, E006AA

Price and Availability

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Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis

Protocols & Articles


Cancer Stem Cell Proliferation in Human Prostate and Breast Cancer Cell Lines Utilizing a New Defined Serum-Free 3D Spheroid Media

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Kan Saito, Nick Asbrock, Vi Chu
Cellular Assays, Biological Reagents & Kits, MilliporeSigma, Temecula, CA
Keywords: Cancer, Cell culture, Culture media, Detection methods, Diffusion, Flow cytometry, Gene expression, Metabolites

Cancer Stem Cells: New Targets for Cancer Therapy

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Keywords: Cancer, Cell attachment, Cell culture, Cell proliferation, Gastrointestinal, Gene expression, Transcription, transformation


Cancer Stem Cell Tumorsphere Formation Protocol

Solid tumors grow in a three-dimensional (3D) spatial conformation, resulting in a heterogeneous exposure to oxygen and nutrients as well as to other physical and chemical stresses. To mimic the 3D s...
Keywords: Cancer, Cell culture

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