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S3077 Sigma-Aldrich

3dGRO Spheroid Medium

Synonym: CSC media, Cancer stem cell media, tumorsphere culture media, tumorsphere media



Related Categories 3D Cell Culture Products, 3D Stem Cell Culture Products, Cell Culture Supplies, Labware
packaging   pkg of 250 mL (basal medium)
shipped in   wet ice
storage temp.   2-8°C


General description

Cancer stem cell (CSCs) are defined as a small subset of cells within a tumor with the ability to self-renew and often drive tumor progression and recurrence after chemotherapy treatment. Therefore, development of specific therapies targeted at CSCs holds hope for improvement of survival and quality of life of cancer patients, especially for patients with metastatic disease.
Traditionally, cancer stem cells have been isolated from cancer cell lines and tumor biopsies and grown in undefined serum containing media using 3-dimensional cell cultures. Using these undefined culture conditions leads to experimental variability and suboptimal cell growth.
3dGRO Spheroid Medium is a new chemically defined and serum-free cancer stem cell media that supports the expansion of CSC′s in 3D spheroid cell cultures. The media supports serial passaging of cancer cell lines leading to an overall enrichment of CSC populations.

Features and Benefits

• Chemically defined, Serum-free formulation.
• High tumorsphere formation efficiency.
• Allows extended serial tumorsphere passaging (>10).
• Proven to enrich CSCs from numerous cancer cell lines.

Storage and Stability

Upon receipt, the basal medium can be stored at 4° C and the vial of supplement can be stored at -20° C stably until the expiration date.

Before first use, the the entire contents of the supplement vial should be added to the basal medium. The shelf life of the complete medium after addition of the supplement is 6 weeks when stored at 4° C. It is recommended to aliquot and prewarm only the required amount of complete medium for each use and to continue to store the remainder at 4° C.

Other Notes

Cell Lines tested with 3dGRO Spheroid Medium:
MCF-7, A-431, Panc-1, LNCaP, HT-29, A-549, U-87 MG, HT1080, E006AA

Legal Information

3dGRO is a trademark of Merck KGaA, Darmstadt, Germany

Safety & Documentation

Safety Information

Hazard statements 
NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Protocols & Articles


Cancer Stem Cells: New Targets for Cancer Therapy

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Overview of 3D Cell Culture: Tools and Techniques

Cell culture techniques are ubiquitous in areas of developmental biology, drug discovery, regenerative medicine and protein production. Since the introduction of cell culture techniques, cells have b...
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Serum-Free 3D Spheroid Media

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Kan Saito, Nick Asbrock, Vi Chu
Cellular Assays, Biological Reagents & Kits, MilliporeSigma, Temecula, CA
Keywords: Cancer, Cell culture, Culture media, Detection methods, Diffusion, Flow cytometry, Gene expression, Metabolites


Cancer Stem Cell Tumorsphere Formation Protocol

Solid tumors grow in a three-dimensional (3D) spatial conformation, resulting in a heterogeneous exposure to oxygen and nutrients as well as to other physical and chemical stresses. To mimic the 3D s...
Keywords: Cancer, Cell culture

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