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S400HR Sigma-Aldrich

Sephacryl®

400-HR, MW range 20-8000 kDa (globular proteins)

Synonym: Poly([allyl dextran]-co-N,N′-methylenebisacrylamide)

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Properties

Related Categories Analytical/Chromatography, Gel Filtration Chromatography, Gel Filtration Media, Molecular Biology, Protein Chromatography,
form   suspension (20% aqueous ethanol)
feature   autoclavable
  stability stable to 0.2M NaOH
matrix   crosslinked allyl dextran/N,N′-methylenebisacrylamide copolymer
particle size   25-75 μm (wet)
pore size   10-2000 kDa MW range (dextrans)
  20-8000 kDa MW range (globular proteins)
compatibility   mode of use size exclusion chromatography
storage temp.   2-8°C

Description

General description

• Supplied swollen, suspended in 20% aqueous ethanol
• Autoclavable
• Stable to 0.2N sodium hydroxide (NaOH)
• Store at refrigerated temperature (2-8 °C)

S400HR-750ML′s updated product is GE17-0609-01

Application

Sephacryl® is used in gel filtration chromatography, protein chromatography, and resins and separation media. Sephacryl® has been used to develop a novel high-throughput screening assay for sickle cell disease drug discovery. Sephacryl® has also been used to purify and characterize enzymes such as bovine pancreatic elastase, tissue-nonspecific alkaline phosphatases and a lysozyme from human urine.

For size exclusion chromatography; the HR grade is a smaller particle size with narrower size range distribution, allowing for more efficient separations at faster flow rates. Recommended fractionation ranges are listed for globular proteins and dextrans.

Packaging

100, 250, 750 mL in glass bottle

Legal Information

Sephacryl is a registered trademark of GE Healthcare

Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Warning
Hazard statements 
RIDADR 
NA 1993 / PGIII
WGK Germany 
1
Flash Point(F) 
100.4 - 109.4 °F
Flash Point(C) 
38 - 43 °C

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Selection Guide for Polymeric Resins in Chromatography & Purification

Sigma-Aldrich carries a vast array of resins, from leading manufacturers; such as, Dow Chemical, Rohm & Haas, Mitsubishi, and Lanxess. Our product line includes adsorbent, ion-exchange (anion / catio...
Keywords: Adsorption, Antibiotics, Combinatorial synthesis, Detergents, Fermentation, Filtration, Hormones, Ion Exchange, Ion exchange resins, Pharmaceutical, Purification, Separation

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

GE17-0609-01 Sephacryl® S-400 HR, GE Healthcare, 17-0609-01, pack of 750 mL
08168 Timestrip Plus 8 °C

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