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S4014 Sigma-Aldrich

Spectinomycin dihydrochloride pentahydrate

powder, BioReagent, suitable for cell culture



Related Categories Aminoglycosides, Antibacterial, Antibiotics, Antibiotics A to Z, Antibiotics N-S,
biological source   synthetic (inorganic)
product line   BioReagent
assay   ≥98.0%
form   powder
potency   ≥603 μg per mg
application(s)   cell culture | plant: suitable
impurities   endotoxin, tested
color   white to off-white
solubility   H2O: soluble 50 mg/mL at 
suitability   suitable for cell culture
Featured Industry   Agriculture
Mode of action   protein synthesis | interferes
antibiotic activity spectrum   Gram-negative bacteria
  Gram-positive bacteria
storage temp.   2-8°C


Biochem/physiol Actions

Mode of Action: Spectinomycin acts by inhibiting protein synthesis and elongation by binding to the bacterial 30S ribosomal subunit and interfering with peptidyl tRNA translocation.

Mode of Resistance: A mutation in rpsE, the gene for ribosomal protein S5, prevents the binding of spectinomycin. Resistance is also conferred by the aminoglycoside-3′-adenyltransferase gene (aadA).

Antimicrobial spectrum: Spectinomycin acts against gram-negative and gram-positive bacteria.


Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.


5, 25 g in poly bottle

Preparation Note

Stock solutions should be prepared at 10 mg/mL in water and filter sterilized.


Spectinomycin dihydrochloride pentahydrate has been used as a supplement in different types of media.

General description

Chemical structure: aminoglycoside

Spectinomycin has generally been used for the treatment of gonorrhea in males, and a study conducted shows that spectinomycin dihydrochloride pentahydrate provides effective treatment against gonorrhea as well. Clinically, spectinomycin dihydrochloride pentahydrate is easier to use in injections as it shows higher solubility than spectinomycin.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Request a Serum Sample

Cell Culture Guide
Protocols & Articles

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