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S5150 Sigma-Aldrich

Sodium chloride solution

5 M in H2O, BioReagent, for molecular biology, suitable for cell culture

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Properties

Related Categories Biochemicals, Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions,
Quality Level   200
grade   for molecular biology
sterility   0.2 μm filtered
product line   BioReagent
concentration   5 M in H2O
application(s)   cell culture | mammalian: suitable
density   1.190 g/cm3
anion traces   phosphate (PO43-): ≤5 ppm
cation traces   K: ≤50 ppm
  heavy metals (as Pb): ≤5 ppm
Featured Industry   Agriculture
foreign activity   DNAse, Exonuclease; RNAse; NICKase, Endonuclease; Protease, none detected
SMILES string   [Na+].[Cl-]
InChI   1S/ClH.Na/h1H;/q;+1/p-1
InChI key   FAPWRFPIFSIZLT-UHFFFAOYSA-M

Description

Application

Sodium chloride solution has been used as a component of mycobacterial lysis buffer to extract DNA. It has also been used along with Dulbecco′s phosphate-buffered saline (DPBS) in the preparation of degassed buffer to elute plasma samples.

Preparation Note

Prepared in 18 megohm water.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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pHast Pack
Protocols & Articles

Protocols

Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

1.    Grow cells to ~80% confluency.  You will need ~2 million cells/RIP, or ~4 million cells for IgG (neg control) & Ago2 RIP
Keywords: Cell disruption, Immunoprecipitation, Individual protein Immunoprecipitation, RNA immunoprecipitation

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Peer-Reviewed Papers
15

References

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