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S5150 Sigma

Sodium chloride solution

5 M in H2O, BioReagent, for molecular biology, suitable for cell culture

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Properties

Related Categories Biochemicals, Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions,
grade   BioReagent
  for molecular biology
InChI Key   FAPWRFPIFSIZLT-UHFFFAOYSA-M
sterility   0.2 μm filtered
concentration   5 M in H2O
density   1.190 g/cm3
anion traces   phosphate (PO43-): ≤5 ppm
cation traces   K: ≤50 ppm
  heavy metals (as Pb): ≤5 ppm
suitability   suitable for cell culture
Featured Industry   Agriculture
foreign activity   DNAse, Exonuclease; RNAse; NICKase, Endonuclease; Protease, none detected

Description

Preparation Note

Prepared in 18 megohm water.

Price and Availability


Biomedical Applications

Webinar: Biological Buffers
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Protocols

Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

1.    Grow cells to ~80% confluency.  You will need ~2 million cells/RIP, or ~4 million cells for IgG (neg control) & Ago2 RIP
Keywords: Cell disruption, Immunoprecipitation, Individual protein Immunoprecipitation, RNA immunoprecipitation

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Peer-Reviewed Papers
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References

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