• USA Home
  • S5188 - Anti-SGK antibody produced in rabbit

S5188 Sigma

Anti-SGK antibody produced in rabbit

buffered aqueous solution, IgG fraction of antiserum



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Primary Antibodies,
species reactivity   human
application(s)   indirect immunofluorescence: 1:2,000 using 3% paraformaldehyde-fixed, 0.5% Triton X-100 treated human epidermal carcinoma A431cell line
  microarray: suitable
  western blot: 1:2,000 using whole cell extract of the human epidermal carcinoma A431 cell line
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 50 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... SGK1(6446)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to the C-terminus of human SGK (amino acids 412-431).

General description

SGK (serum and glucocorticoid-regulated protein kinase) is a 50 kDa member of Ser/Thr kinase family. It is regulated at the transcription level by stimuli including glucocorticoids, serum, follicle stimulating hormone as well as changes in cell volume. Sequence of sgk is well conserved across species, the human and rat SGK amino acid sequences are 96-98% identical. SGK plays an important role in signal transduction activated by mineral corticoids. SGK is transcriptionally regulated by the glucocorticoid response element present in sgk promoter. SGK levels are strongly affected by osmotic changes that in changes in the cell volume. SGK is strongly and rapidly stimulated in kidney cells in response to aldosterone, and stimulates epithelial Na+ channels, suggesting a central role of SGK in the regulation of sodium transport and homeostasis. SGK transcription is markedly increased in diabetic nephropathy, in response to excessive extracellular glucose concentrations. The localization of SGK in the cell is regulated by hormones or serum. For cell cycle progression, the shuttling of SGK between nucleus and cytoplasm is suggested as a requirement.
Anti-SGK recognizes human SGK (50 kDa, appearing as a doublet).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide


Anti-SGK antibody can be used at a minimum working dilution of 1:2,000 for detection by immunoblotting in whole extract of the human epidermal carcinoma A431 cell line. Indirect can be performed on human epidermal carcinoma A431 cell line at a working dilution of 1:2,000. The antibody is suitable for protein microarray.

Price and Availability

Antibody Explorer

Explore with Confidence
Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?