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S5193 Sigma-Aldrich

SYBR® Green JumpStart Taq ReadyMix

for quantitative PCR, without MgCl2 in buffer

Purchase

Properties

Related Categories Biochemicals and Reagents, Core Bioreagents, Fluorescent Kits, Fluorescent Probes, Labels, Particles and Stains, Life Science Reagents for PCR,
form   liquid
feature   hotstart
concentration   1.25 units/reaction (50 μL reaction volume)
color   colorless
Featured Industry   Agriculture
compatibility   for use with ABI 5700
  for use with ABI 7000
  for use with ABI 7300
  for use with ABI 7500 Fast
  for use with ABI 7500
  for use with ABI 7700
  for use with ABI 7900 Fast
  for use with ABI 7900 HT
  for use with ABI 7900
  for use with ABI StepOne
  for use with ABI StepOnePlus
  for use with ABI ViiA 7
  for use with Bio-Rad CFX384
  for use with Bio-Rad CFX96
  for use with Bio-Rad MJ Chromo4
  for use with Bio-Rad MJ Opticon 2
  for use with Bio-Rad MJ Opticon Cepheid SmartCycler
  for use with Bio-Rad MJ Opticon
  for use with Bio-Rad MiniOpticon
  for use with Eppendorf® Mastercycler ep realplex2 s
  for use with Eppendorf® Mastercycler ep realplex
  for use with Illumina Eco qPCR
  for use with Qiagen Corbett Rotor-Gene 3000
  for use with Qiagen Corbett Rotor-Gene 6000
  for use with Qiagen Corbett Rotor-Gene Q
  for use with Roche LightCycler 480
  for use with Strategene Mx3000P
  for use with Strategene Mx3005P
  for use with Strategene Mx4000
shipped in   wet ice
storage temp.   −20°C

Description

Features and Benefits

• Delivers the benefits of antibody-inactivated hot-start PCR with SYBR Green detection in a ReadyMix ideal for high throughput applications; only primers and template are required.
• JumpStart Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield.
• SYBR Green JumpStart Taq ReadyMix for SYBR based QPCR is formulated with MgCl2 or packaged with a separate vial for ease of optimization. ReadyMixes are compatible with tube- and plate-based instruments.

Packaging

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
400RXN is packaged as 1 X 10 mL
500RXN is packaged as 1 X 12.5 mL

Other Notes

Magnesium chloride is provided separately for optimization.

SYBR Green JumpStart Taq ReadyMix combines the performance enhancements of JumpStart Taq antibody for hot start PCR with SYBR Green I and the convenience of an easy-to-use ReadyMix solution. This ready-to-use mixture of SYBR Green I, JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer is provided in a 2× concentrate for ease of use. Simply add 25 μL of the 2× mix to DNA template, primers and water. The JumpStart Taq antibody inactivates the DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate CT values.

Principle

SYBR Green JumpStart Taq ReadyMix is recommended for single product real-time amplification experiments and can also be used for PCR optimization prior to manufacture of fluorescent-labeled probes. Fluorescent labeled probes are not recommended for use with SYBR Green I dye.

SYBR Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. SYBR Green I has an excitation and emission maxima of 494 nm and 521 nm, respectively. The instrument settings for ROX reference dye are satisfactory for the measurement of the Reference Dye for Quantitative PCR. Specificity of Sigma′s SYBR based QPCR detection is greatly enhanced by the incorporation of a hot-start mediated taq polymerase, JumpStart Taq.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

Eppendorf is a registered trademark of Eppendorf AG

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

SYBR is a registered trademark of Life Technologies

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Protocols

Universal SYBR Green Quantitative PCR Protocol

Technology Overview Assay Considerations Methods of Quantification Equipment & Supplies PCR Mix Selection Guide Protocol Troubleshooting Materials References
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Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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