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S5395 Sigma-Aldrich

Superoxide Dismutase from bovine erythrocytes

≥3,000 units/mg protein, BioReagent, lyophilized powder, suitable for cell culture

Synonym: SOD, Superoxide: superoxide oxidoreductase

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Description

General description

Superoxide Dismutase from bovine erythrocytes is a metalloprotein which disproportionates superoxide anion radicals. It is a 31.5 kDa copper binding protein and displays a conserved domain and fold. It is a homodimer with one copper and zinc ion per subunit and has antiparallel “greek-key” β barrel fold.

Application

Superoxide Dismutase (SOD) from bovine erythrocytes has been used:
• for measuring the superoxide radical using the electron paramagnetic resonance spin in human brain microvascular endothelial cells
• for measuring superoxide production in cytochrome C assay in peripheral blood mononuclear cells
• as a standard in characterization of hen egg SOD using Fourier-transform infrared spectroscopy (FTIR) and matrix-assisted laser desorption/ionization (MALDI) analysis

Packaging

15000 units in glass bottle

30000, 75000 units in poly bottle

Biochem/physiol Actions

Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Unit Definition

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.

Analysis Note

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

HPLC Analysis of Superoxide Dismutase on Discovery® BIO PolyMA-WAX

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Superoxide Dismutase on Discovery® BIO PolyMA-WAX
Keywords: Chromatography, High performance liquid chromatography

Protocols

Enzymatic Assay of Superoxide Dismutase

This procedure applies to all products that have a specification for superoxide dismutase activity by enzymatic determination.
Keywords: Catalysis, Reductions

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Peer-Reviewed Papers
15

References

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