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S5567 Sigma-Aldrich

SP600125

≥98% (HPLC)

Synonym: 1,9-Pyrazoloanthrone, Anthrapyrazolone

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Properties

Related Categories Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules, Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology,
Quality Level   200
assay   ≥98% (HPLC)
color   yellow
solubility   DMSO: 20 mg/ml, clear to very slightly hazy, faintly yellow to yellow
  H2O: insoluble
storage temp.   2-8°C
SMILES string   O=C1c2ccccc2-c3n[nH]c4cccc1c34
InChI   1S/C14H8N2O/c17-14-9-5-2-1-4-8(9)13-12-10(14)6-3-7-11(12)15-16-13/h1-7H,(H,15,16)
InChI key   ACPOUJIDANTYHO-UHFFFAOYSA-N
Gene Information   human ... JUN(3725), MAPK10(5602), MAPK9(5601)

Description

Application

C2C12 myoblasts were treated with SP600125 to test the stimulation of myogenesis.11 It was used to treat HepG2 cells to test the effects on oxysterol-induced necrosis.12

Biochem/physiol Actions

SP600125 is an anthrapyrazolone inhibitor of JNK that competes with ATP to inhibit the phosphorylation of c-Jun. It prevents the activation of inflammatory genes such as COX-2, IL-2 IFN-γ and TNF-α.8,9 It prevents the activation of JNK after brain ischemia and may be effective in treatment of ischemic stroke.10

A novel and selective inhibitor of c-Jun N-terminal kinase (JNK)

Features and Benefits

This compound is also offered as part of Sigma′s Library of Pharmacologically Active Compounds (LOPAC®1280), a biologically annotated collection of high-quality, ready-to-screen compounds. Click here to learn more.

Legal Information

LOPAC is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
CB4585000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

EGF Signaling: Tracing Cancer's Path

The epidermal growth factor (EGF) family is a group of structurally related proteins that regulate cell proliferation, migration and differentiation via tyrosine kinase receptors on target cells. The...
Keywords: Apoptosis, Cancer, Cell proliferation, Cloning, Gene expression, Growth factors, Ligands, Morphogenesis, Phosphorylations, Transcription, Transduction, transformation

Naive Pluripotent Stem Cell Culture in an Inhibitor Based 2i/LIF Containing Serum-Free and Feeder-Free Stem Cell Media

Naive pluripotent stem cells are located within the epiblast of mature blastocysts. These primitive “ground-state” cells may be cultured in vitro using specialized media and small molecule inhibitors...
Gabi Tremml, Ming Li and Vi Chu1*,
1MilliporeSigma, Bioscience Division, Temecula, CA, USA
 
Keywords: Cell culture, Gene expression, Immunocytochemistry, Microscopy, Polymerase chain reaction, Polymerase chain reaction - quantitative, Transcription

The Role of Inflammation in Neurodegeneration

It is well established that the brain can sense and react to peripheral insults through the rapid induction of fever and other neuroendocrine changes in response to events such as infection and tissu...
Scott Hauser, Technology Transfer Specialist, Sigma® Life Science
Biofiles, Vol. 8, No. 19
Keywords: Adhesion, Central Nervous System, Diseases, Gene expression, Inflammation, Neurodegenerative Diseases, Reductions

Peer-Reviewed Papers
15

References

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