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S5569 Sigma-Aldrich

Anti-Superoxide Dismutase (MnSOD) (KC-19) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Indophenoloxidase B, Anti-IPO-B, Anti-MNSOD, Anti-Manganese Dependent SOD, Anti-Manganese Superoxide Dismutase 2, Anti-Mitochodrial SOD, Anti-Mn-SOD, Anti-SOD2, Anti-Superoxide Dismutase 2

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Cell and Organelle Proteins,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect immunofluorescence: 1:250-1:500 using methanol-acetone-fixed rat NRK cells
  western blot: 1:1,000-1:2,000 using whole extract of rat brain and a chemiluminescent detection reagent
  western blot: 1:2,000-1:4,000 using human HeLa mitochondria extract and a chemiluminescent detection reagent
species reactivity   rat, human
mol wt   antigen ~24 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P04179
Gene Information   human ... SOD2(6648)
mouse ... Sod2(20656)
rat ... Sod2(24787)

Description

General description

Manganese superoxide dismutase (MnSOD) protein is a homotetrameric SOD. This protein is composed of four subunits, each containing one Mn2+. MnSOD enzyme is synthesized in the cytosol and is modified for its transport into the mitochondria where it resides in the matrix.

The SOD2 (superoxide dismutase 2) gene is mapped to human chromosome 6q25.3. The encoded protein functions as a tetramer and is localized to mitochondria.

Specificity

Anti-Superoxide Dismutase (MnSOD) (KC-19) recognizes human and rat Superoxide Dismutase (MnSOD).

Immunogen

synthetic peptide corresponding to amino acid residues 202-220 of human superoxide dismutase (MnSOD), conjugated to KLH. The corresponding sequence is identical in apes and monkeys, differs by one amino acid in mouse and horse, by two amino acids in cow, and by four amino acids in rat.

Application

Anti-Superoxide Dismutase (MnSOD) (KC-19) antibody produced in rabbit may be used in immunoblotting and indirect immunofluorescence.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Manganese superoxide dismutase (MnSOD) is essential for the survival of aerobic life. This protein is inducible by tumor necrosis factor (TNF) and protects cells from TNF-mediated apoptosis. MnSOD is proposed to function as a tumor suppressor by modulating the activity of redox-sensitive transcription factors and specific signal mediators. Overexpression of the MnSOD gene protects neurons from N-methyl-D-aspartate receptor (NMDA) and nitric oxide-induced neurotoxicity. A decline in the MnSOD activity occurs in aging, progeria, cancer, asthma and transplant rejection.

Superoxide Dismutase 2 (SOD2) is an antioxidant enzyme and catalyzes the conversion of superoxide to hydrogen peroxide and molecular oxygen. The enzyme offers protection against oxidative damage induced by reactive oxygen species and also is radioprotective. The gene is associated with the metastasis and invasion process of salivary adenoid cystic carcinoma. Manganese SOD is involved in detoxification.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

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Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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