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S5761 Sigma-Aldrich

Sodium bicarbonate

powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture

Synonym: Sodium hydrogen carbonate

  • CAS Number 144-55-8

  • Linear Formula NaHCO3

  • Molecular Weight 84.01

  •  Beilstein/REAXYS Number 4153970

  •  EC Number 205-633-8

  •  MDL number MFCD00003528

  •  PubChem Substance ID 24899673

  •  NACRES NA.75

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Properties

Related Categories Apoptosis and Cell Cycle, Biological Buffers, Buffers, Cell Biology, Cell Culture,
Quality Level   300
grade   for molecular biology
product line   BioReagent
assay   99.5-100.5%
form   powder
quality   Premium
application(s)   cell culture | insect: suitable
  cell culture | mammalian: suitable
pH range   6.8 - 7.4
pKa (25 °C)   (1) 6.37, (2) 10.25 (carbonic acid)
solubility   water: soluble 100 mg/mL
density   2.16 g/mL at 25 °C (lit.)
SMILES string   [Na+].OC([O-])=O
InChI   1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
InChI key   UIIMBOGNXHQVGW-UHFFFAOYSA-M

Description

General description

Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications.

Application

Sodium bicarbonate has been used:
• as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)
• as a component of a 1:1 mixture of Dulbecco′s modified Eagle′s medium and Ham′s nutrient mixture F-12 during the culture of bovine microvascular endothelial cells
• as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cells
• as a component of medium used for cell culture of human embryonic kidney cells
• as a component of Beltsville thawing solution used for the dilution of semen

Packaging

1 kg in poly bottle

5 kg in poly drum

500 g in poly bottle

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
VZ0950000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Screening for Estrogen Active Nonylphenols in Water by Planar Solid Phase Extraction – Planar Yeast Estrogen Screen

Nonylphenols (NP) are ubiquitous substances that have been detected in highly diverse foodstuff and that inter alia show estrogenic activity.1,2 NP can presumably end up in the aquatic environment as...
Keywords: Chromatography, Environmental, Hormones, Solid phase extractions

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Life Science Materials

With a broad portfolio of widely-cited reagents and kits supported by industry-leading expertise, we help you get the most out of your life science research.

Peer-Reviewed Papers
15

References

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Description

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S8761 Sodium bicarbonate solution, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture
S4019 Sodium bicarbonate, Hybri-Max, powder, suitable for hybridoma, ≥99.5%

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