S5761 Sigma-Aldrich

Sodium bicarbonate

powder, BioReagent, for molecular biology, suitable for cell culture, suitable for insect cell culture

Synonym: Sodium hydrogen carbonate

  • CAS Number 144-55-8

  • Linear Formula NaHCO3

  • Molecular Weight 84.01

  •  Beilstein/REAXYS Number 4153970

  •  EC Number 205-633-8

  •  MDL number MFCD00003528

  •  PubChem Substance ID 24899673

  •  NACRES NA.75



Related Categories Apoptosis and Cell Cycle, Biological Buffers, Buffers, Cell Biology, Cell Culture,
Quality Level   300
grade   for molecular biology
product line   BioReagent
assay   99.5-100.5%
form   powder
quality   Premium
application(s)   cell culture | insect: suitable
  cell culture | mammalian: suitable
pH range   6.8 - 7.4
pKa (25 °C)   (1) 6.37, (2) 10.25 (carbonic acid)
solubility   water: soluble 100 mg/mL
density   2.16 g/mL at 25 °C (lit.)
SMILES string   [Na+].OC([O-])=O
InChI   1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1


General description

Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. It is broadly used as an physiological buffer in in vitro applications.


Sodium bicarbonate has been used:
• as a component of bovine embryo culture system during in vitro maturation, fertilization, and culture (IVMFC)
• as a component of a 1:1 mixture of Dulbecco′s modified Eagle′s medium and Ham′s nutrient mixture F-12 during the culture of bovine microvascular endothelial cells
• as a component of ITS (insulin transferrin and selenite) medium used during the isolation of dopamine neurons from human embryonic stem cells
• as a component of medium used for cell culture of human embryonic kidney cells
• as a component of Beltsville thawing solution used for the dilution of semen


1 kg in poly bottle

5 kg in poly drum

500 g in poly bottle

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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S8761 Sodium bicarbonate solution, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture
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