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S5933 Sigma-Aldrich

Anti-STAT3 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Proteins/Bioactives/Markers/Receptors for Stem Cell Biology, Antibodies for Cell Biology, Antibodies for Embryonic Stem Cells,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: 10 μg/mL using A431 cells
  immunoprecipitation (IP): 4 μg using STAT3 from 0.5–1 mg of an EGF stimulated human A431 cell lysate
  western blot (chemiluminescent): 0.5-2 μg/mL using lysates from EGF stimulated human A431, mouse WEHI and rat L6 cells
species reactivity   rat, mouse, human
mol wt   antigen 92 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
UniProt accession no.   P40763
Gene Information   human ... STAT3(6774)
mouse ... Stat3(20848)
rat ... Stat3(25125)

Description

Specificity

The antibody is specific for STAT3. It may detect an additional 66 kDa band using high concentrations or long exposures. The sequence recognized by the antibody (688-722) is identical in mouse and rat STAT3. The first 28 amino acids are identical to mouse STAT3B.

Immunogen

GST fusion protein corresponding to amino acids 688-722 of human STAT3, expressed in bacteria.

Physical form

Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M NaCl and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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