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S6322 Sigma

Sigma Adjuvant System®




Frequently Asked Questions

Frequently Asked Questions are available for this Product.


Adjuvanticity Assay: EIA titer for test mice is at least 2X greater that that of control mice.

Biochem/physiol Actions

Sigma Adjuvant System is for use in mice. It is a stable oil-in-water emulsion that may be used as an alternative to the classical Freund′s water-in-oil emulsions. This adjuvant is derived from bacterial and mycobacterial cell wall components that provide potent stimulus to the immune system.

Physical form

Each vial contains 0.5 mg Monophosphoryl Lipid A (detoxified endotoxin) from Salmonella minnesota and 0.5 mg synthetic Trehalose Dicorynomycolate in 2% oil (squalene)-Tween® 80-water.

Legal Information

Sigma Adjuvant System is a registered trademark of Sigma-Aldrich Co. LLC

TWEEN is a registered trademark of Croda International PLC

Price and Availability

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Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Is the Sigma Adjuvant System®, Product No S6322, the same as the RIBI adjuvant?
Yes, the Sigma Adjuvant System®, Product No S6322, is also called RIBI adjuvant. 
What is used to solubilize Product No. S6322, Sigma Adjuvant System® reagent?
The Sigma Adjuvant System® reagent (Product No. S6322) can be solubilized in a sterile saline solution containing the desired amount of antigen to a final volume of 2 ml.  For more details, please see the S6322 Product Information Sheet.
Can you reconstitute and store the Sigma Adjuvant System® reagent (Product No. S6322) if not using the entire vial right away?
If the entire contents of the vial will not be used initially, reconstitute with 1 ml saline without antigen.  This emulsion can then be stored at 2-8 °C for up to 60 days.  Do Not Freeze. To use, mix aliquots 1:1 with antigen in saline. Prior to inoculation, warm the vial to 37 °C and vortex briefly.  This and other information can be found on the Sigma Adjuvant System® reagent (Product No. S6322) Product Information Sheet.
When using the Sigma Adjuvant System®, Product No. S6322, how is the antigenic response boosted?
With the Sigma Adjuvant System® (Product No. S6322) with mice, rabbits, rats and guinea pigs; boost on day 21 or 28, bleed 10 - 14 days after booster injection. Boost every 3 - 4 weeks thereafter. For goats; boost every four weeks. Use the same protocol for each injection.  For more information please see the Product Information Sheet for Product No. S6322.
When using the Sigma Adjuvant System® reagent, Product No. S6322, how do the components MPL and TDM work as an adjuvant?
MPL (monophosphoryl lipid A) will bind to CD14 on the cell surface similar to LPS.  TDM (trehalose dicorynomycolate) is an analog of one of the components of the tubercle bacillus. Both of these components act like a superantigen and activate the immune system nonspecifically.  This allows for a greater response to the immunogen delivered in this adjuvant.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
Has Sigma Adjuvant System®, Product S6322, been used to induce EAE (Experimental autoimmune encephalomyelitis)?
Sigma Adjuvant System®, Product S6322 is a replacement for MPT+TDM adjuvant (Ribi, Product M6536) that was discontinued by the supplier Corixa.  Although we have not tested Product S6322 for induction of Experimental autoimmune encephalomyelitis (EAE), the following reference cites the use of MBP in Ribi adjuvant for induction of acute EAE. The Journal of ImmunologyOctober 15, 2005, 175(8): 5077-5086
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