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S7388 Sigma-Aldrich

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide

Synonym: N-Succinyl-Ala-Ala-Pro-Phe-pNA, N-Succinyl-L-alanyl-L-alanyl-L-prolyl-L-phenylalanine 4-nitroanilide

  • CAS Number 70967-97-4

  • Empirical Formula (Hill Notation) C30H36N6O9

  • Molecular Weight 624.64

  •  Beilstein/REAXYS Number 4289896

  •  MDL number MFCD00077161

  •  PubChem Substance ID 24899742

  •  NACRES NA.32

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Properties

Related Categories Apoptosis Enzymes, Apoptosis and Cell Cycle, Application Index, Biochemicals and Reagents, Cathepsin G,
storage temp.   −20°C
SMILES string   [H][C@]1(CCCN1C(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)CCC(O)=O)C(=O)N[C@@H](Cc2ccccc2)C(=O)Nc3ccc(cc3)[N+]([O-])=O
InChI   1S/C30H36N6O9/c1-18(31-25(37)14-15-26(38)39)27(40)32-19(2)30(43)35-16-6-9-24(35)29(42)34-23(17-20-7-4-3-5-8-20)28(41)33-21-10-12-22(13-11-21)36(44)45/h3-5,7-8,10-13,18-19,23-24H,6,9,14-17H2,1-2H3,(H,31,37)(H,32,40)(H,33,41)(H,34,42)(H,38,39)/t18-,19-,23-,24-/m0/s1
InChI key   LKDMKWNDBAVNQZ-WJNSRDFLSA-N
Gene Information   human ... FKBP10(60681)

Description

Amino Acid Sequence

N-Suc-Ala-Ala-Pro-Phe-pNA

Application

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide has been used as a substrate to determine chymotrypsin activity.

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide has been used:
• as a substrate for cathepsin G assay in bone marrow lysates.
• in proteolytic activity assay of serine protease subtilisin carlsberg (SC).
• as a substrate for neutrophil elastase, trypsin and chymotrypsin in protease assay.

Packaging

25, 100, 250, 500 mg in poly bottle

Biochem/physiol Actions

N-succinyl-Ala-Ala-Pro-Phe p-nitroanilide is a substrate for subtilisin and proteases.

Substrates

N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide is a colorimetric substrate for chymotrypsin, human leukocyte cathepsin G and peptidyl prolyl isomerase.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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