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S7446 Sigma-Aldrich

Superoxide Dismutase from bovine erythrocytes

BioUltra, lyophilized powder, ≥4,500 units/mg protein, ≥97% (SDS-PAGE)

Synonym: SOD, Superoxide: superoxide oxidoreductase

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Properties

Related Categories 1.15.x.x Acting on superoxide radicals, 1.x.x.x Oxidoreductases, Application Index, Biochemicals and Reagents, Cell Signaling Enzymes,
product line   BioUltra
assay   ≥97% (SDS-PAGE)
form   lyophilized powder
composition   Protein, ≥95% biuret
storage temp.   −20°C

Description

General description

Superoxide dismutases are a group of low molecular weight metalloproteins present in all aerobic cells of plants, animals and micro-organisms. They provide protection against damaging reactions with the superoxide radical anion (O2-) by catalyzing its disproportionation into oxygen and hydrogen peroxide.

Application

Superoxide Dismutase from bovine erythrocytes has been used in a study to assess activity parameters in adjuvant-induced arthritis. Superoxide Dismutase from bovine erythrocytes has also been used in a study to simplify the process of purifying superoxide dismutases using charge-controlled hydrophobic chromatography on 10-carboxydecyl Sepharose.

Packaging

15000, 75000 units in glass bottle

Biochem/physiol Actions

Catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. Plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

Unit Definition

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA550 of 0.025 ± 0.005 per min.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Articles

HPLC Analysis of Superoxide Dismutase on Discovery® BIO PolyMA-WAX

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Superoxide Dismutase on Discovery® BIO PolyMA-WAX
Keywords: Chromatography, High performance liquid chromatography

Protocols

Enzymatic Assay of Superoxide Dismutase

This procedure applies to all products that have a specification for superoxide dismutase activity by enzymatic determination.
Keywords: Catalysis, Reductions

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

Related Products

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