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S7903 Sigma-Aldrich

Sucrose

BioXtra, ≥99.5% (GC)

Synonym: α-D-Glc-(1→2)-β-D-Fru, α-D-Glucopyranosyl β-D-fructofuranoside, β-D-Fructofuranosyl-α-D-glucopyranoside, D(+)-Saccharose, Sugar

  • CAS Number 57-50-1

  • Empirical Formula (Hill Notation) C12H22O11

  • Molecular Weight 342.30

  •  Beilstein/REAXYS Number 90825

  •  EC Number 200-334-9

  •  MDL number MFCD00006626

  •  PubChem Substance ID 24899768

  •  NACRES NA.21

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Properties

Related Categories Biochemicals and Reagents, Carbohydrates, Carbohydrates A to Z, Carbohydrates P-Z, Carbohydrates for Protein Stabilization,
Quality Level   300
biological source   sugar cane
product line   BioXtra
assay   ≥99.5% (GC)
form   crystals
optical activity   [α]20/D +66.5±1°, c = 10 in H2O(lit.)
application(s)   gas chromatography (GC): suitable
impurities   Insoluble matter, passes filter test
ign. residue   ≤0.01% (as SO4)
loss   ≤0.2% loss on drying, HV, 18 - 26°C
pH   5.5-7.5 (20 °C, 1 M in H2O)
mp   185-187 °C (lit.)
solubility   H2O: 1 M, clear, colorless
anion traces   chloride (Cl-): ≤0.005%
  sulfate (SO42-): ≤0.005%
cation traces   Al: ≤0.0005%
  As: ≤0.0001%
  Ba: ≤0.0005%
  Bi: ≤0.0005%
  Ca: ≤0.001%
  Cd: ≤0.0005%
  Co: ≤0.0005%
  Cr: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Li: ≤0.0005%
  Mg: ≤0.0005%
  Mn: ≤0.0005%
  Mo: ≤0.0005%
  Na: ≤0.005%
  Ni: ≤0.0005%
  Pb: ≤0.0005%
  Sr: ≤0.0005%
  Zn: ≤0.0005%
absorption   ≤0.07 at 280 in H2O at 1 M
  ≤0.09 at 260 in H2O at 1 M
storage temp.   room temp
SMILES string   OC[C@H]1O[C@H](O[C@]2(CO)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H](O)[C@@H](O)[C@@H]1O
InChI   1S/C12H22O11/c13-1-4-6(16)8(18)9(19)11(21-4)23-12(3-15)10(20)7(17)5(2-14)22-12/h4-11,13-20H,1-3H2/t4-,5-,6-,7-,8+,9-,10+,11-,12+/m1/s1
InChI key   CZMRCDWAGMRECN-UGDNZRGBSA-N

Description

Application

Sucrose may be used to prepare density gradients for cell/organelle separation. The refractive index of the solution will be directly related to the density. In addition, sucrose can be used as a supplement in plant, insect, and bacterial culture media, and in various enzymatic assays.

Packaging

1 kg in poly bottle

250 g in poly bottle

5 kg in poly drum

Preparation Note

Solutions can be autoclaved for 15-20 minutes at a maximum of 121 °C. There will be some hydrolysis to glucose and fructose, depending in part on how rapidly the autoclave comes to the required temperature and pressure. Care must be taken to prevent solutions from caramelizing.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
WN6500000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Three-Dimensional Printing of Tissue Engineering Scaffolds

Helena N. Chia, Ph.D.,1* Benjamin M. Wu, D.D.S, Ph.D.1,2* 1Department of Bioengineering, Henry Samueli School of Engineering University of California, Los Angeles, CA 90095 USA 2Division of Advanced ...
Keywords: Biomaterials, Ceramics, Chromatin immunoprecipitation, Deposition, Diagnostic Imaging, Diffusion, Growth factors, Materials Science, Melting, Polymerization reactions, Solvents

Protocols

Enzymatic Assay of Invertase

This procedure applies to all products that have specification for the enzymatic activity of Invertase at Sigma-Aldrich Saint Louis.
Keywords: Biochemistry, Enzyme activity, Enzymology

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

Peer-Reviewed Papers
15

References

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