S8445 Sigma-Aldrich


wide range, mol wt 6,500-200,000 Da



Related Categories Core Bioreagents, Life Science Reagents for Protein Electrophoresis, Markers, Molecular Biology, Molecular Weight Markers,
Quality Level   200
form   lyophilized powder
usage   sufficient for 10-20 large gels (16 × 18 cm)
  sufficient for 20-30 mini-gels (10 × 10 cm)
   vial sufficient for 50-100 PhastGels
mol wt   6,500-200,000 Da
storage temp.   2-8°C



SigmaMarker has been used as a protein marker in sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE).

SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining.

Other Notes

Protein (mol. wt.)
Aprotinin, bovine lung (6,500)
α-Lactalbumin, bovine milk (14,200)
Trypsin inhibitor, soybean (20,000)
Trypsinogen, bovine pancreas (24,000)
Carbonic anhydrase, bovine erythrocytes (29,000)
Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000)
Ovalbumin, chicken egg (45,000)
Glutamic dehydrogenase, bovine liver (55,000)
Albumin, bovine serum (66,000)
Phosphorylase B, rabbit muscle (97,000)
β-Galactosidase, E. coli (116,000)
Myosin, porcine heart (200,000)

Will yield 12 protein bands with Coomassie Blue staining.


Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 10 mM TCEP, 2% SDS, and 0.01% bromophenol blue.

Legal Information

SigmaMarker is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 

Single- & multi-channel pipettes from BRAND
Protocols & Articles

Related Content

Molecular Weight Markers

Protein molecular weight markers, sometimes identified as protein standards or protein ladders, are used to estimate the molecular weight of proteins of interest, to monitor the progress of electroph...
Keywords: Electrophoresis, Gel electrophoresis, Isoelectric focusing, Molecular biology, PAGE, Polymerase chain reaction, Protein electrophoresis, Separation, Western blot

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?