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S8820 Sigma-Aldrich

SIGMAFAST Protease Inhibitor Tablets

For General Use

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Description

Specificity

Inhibits serine, cysteine, aspartic, and metalloproteases.

Application

Optimized and tested for general use.

SIGMAFAST Protease Inhibitor Tablets has been used to collect tracheae in combination with ice-cold radioimmunoprecipitation assay buffer in a study to determine the tracheal basal cell fate. It has been used to homogenize rat lung tissues for western blot analysis.

Components

AEBSF, 2 mM
Aprotinin, 0.3 μM
Bestatin, 130 μM
EDTA, 1 mM
E-64, 14 μM
Leupeptin, 1 μM

Other Notes

Tableted formulation containing water-soluble protease inhibitors with broad specificity for the inhibition of serine, cysteine, and metalloproteases. Contains 4-(2-aminoethyl)benzenesulfonyl fluoride (AEBSF), E-64, bestatin, leupeptin, aprotinin, and EDTA (sodium salt).

Quantity

One tablet makes 100 mL of cocktail. One mL is recommended for the inhibition of proteases equivalent to 1 mg of USP pancreatin. One tablet is recommended for the inhibition of proteases present in a maximum of 20 g of cell extract.

Physical form

Tablet that is water soluble.

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the difference between Protease Inhibitors S8820 and P2714?
S8820 is the tablet version of P2714.
CanProduct S8820, SIGMAFAST Protease Inhibitor Tablets, be used with with HIS-Select products or any other immobilized metal affinity chromatography (IMAC) products?
The tablets contain EDTA, a metal chelator, which may remove metal ions from these affinity products. The suggested protease inhibitor for the HIS-Select products is either Product No. S8830 (water soluble tablet) or P8849 (DMSO solution).
Can Product S8820, SIGMAFAST Protease Inhibitor Tablets, be used with ELISA?
Yes, because it is water based.
How should I dissolve Product S8820, SIGMAFAST Protease Inhibitor Tablets?
You can dissolve the product in 10 ml of water to make a 10X stock solution and then dilute 1:10 for use.
What is the concentration of the inhibitors in Product S8820, SIGMAFAST Protease Inhibitor Tablets?
When reconstituted (one tablet in 100 ml water), concentrations of the component inhibitors are as follows:AEBSF2 mME-6414 μMBestatin130 μMLeupeptin0.9 μMAprotinin0.3 μMEDTA1 mM
How much of Product S8820, SIGMAFAST Protease Inhibitor Tablets, needs to be used in cell lysates?
One tablet is recommended for the inhibition of proteases present in a maximum of 20 grams of cell extract.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions
Protocols & Articles

Articles

Protease Inhibitor Cocktails References

Our portfolio of protease inhibitor cocktails are frequently cited in peer-reviewed scientific literature, suggesting their superior quality and excellent track record. This page lists a sampling of ...
Keywords: AGE, Adhesion, Aerobic, Affinity chromatography, Alzheimer Disease, Amplification, Angiogenesis, Anti-inflammatory agents, Antibiotics, Antimicrobials, Antitumor agents, Antivirals, Apoptosis, Atomic absorption spectroscopy, Autophagy, Biochemistry, Biological processes, Buffers, Cancer, Cardiovascular, Catalysis, Cell biology, Cell disruption, Cell proliferation, Cell signaling, Cellular processes, Central Nervous System, Chromatin immunoprecipitation, Chromatography, Clinical, Cloning, Cytotoxins, DNA replication, Degradations, Detergents, Diabetes, Diagnostic, Diseases, Dopamine agents, Drug discovery, Eliminations, Environmental, Enzyme-linked immunosorbent assay, Gas chromatography, Gastrointestinal, Gene expression, Genetic, Genetics, Gluconeogenesis, Glycosylations, Growth factors, Hormones, Hydration reaction, Hydroxylations, Immobilization, Immunology, Immunoprecipitation, Inflammation, Ligands, Lipid Metabolism, Lipid peroxidation, Mass spectrometry, Medicinal chemistry, Metabolism, Metabolites, Methylations, Microarray Analysis, Microbiology, Microscopy, Molecular biology, Morphogenesis, Neurology, Neuroscience, Neurotransmission, Neurotransmitters, Normal-phase chromatography, Nutrition, Obesity, Oncology, PAGE, Parkinson Disease, Peroxidations, Pharmaceutical, Phosphorylations, Photosynthesis, Physiological Processes, Polymerase chain reaction, Polymorphisms, Proteomics, Purification, Recombination, Reductions, Reproduction, Respiratory, Reversible addition-fragmentation chain transfer polymerizations, Sample preparations, Scanning electron microscopy, Schizophrenia, Separation, Sequencing, Sonication, Transcription, Transduction, Veterinary, Vitamins, Western blot, transformation

Peer-Reviewed Papers
15

References

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P8465 Protease Inhibitor Cocktail powder, for use with bacterial cell extracts, lyophilized powder
P8340 Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
P8215 Protease Inhibitor Cocktail, for use with fungal and yeast extracts, DMSO solution
P2714 Protease Inhibitor Cocktail powder, for general use, lyophilized powder S8820 is a tablet version of the P2714 powder.
P8849 Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution
P9599 Protease Inhibitor Cocktail, for plant cell and tissue extracts, DMSO solution
P1860 Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution
S8830 SIGMAFAST Protease Inhibitor Cocktail Tablets, EDTA-Free, for use in purification of Histidine-tagged proteins
08168 Timestrip Plus 8 °C

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