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S8884 Sigma-Aldrich

Safranin O

certified by the Biological Stain Commission

Synonym: Basic Red 2, Cotton Red, Gossypimine, Safranin T, Safranin Y or A

  • CAS Number 477-73-6

  • Empirical Formula (Hill Notation) C20H19ClN4

  • Molecular Weight 350.84

  •  Colour Index Number 50240

  •  Beilstein/REAXYS Number 3924099

  •  EC Number 207-518-8

  •  MDL number MFCD00011759

  •  PubChem Substance ID 24899820

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Properties

Related Categories Cell Biology, Certified Stains & Dyes, Core Bioreagents, Hematology and Histology, Research Essentials,
grade   certified by the Biological Stain Commission
InChI Key   OARRHUQTFTUEOS-UHFFFAOYSA-N
form   powder
composition   Dye content, ≥80%
solubility   H2O: 1 mg/mL

Description

General description

Safranin O is a metachromatic, cationic dye. It is used as a counterstain in Gram staining. The stain colors Gram-negative bacteria pink to red and has no effect on Gram-positive bacteria. It is also used to assess glycosaminoglycans (GAGs) in cartilage. The dye imparts an intense red to GAGs. It easily binds to polyanions.

Application

Safranin O has been used in the staining of extraocular muscle mesenchymal stem cells (EOM-MSC) for the detection of cartilage, mucin and mast cell granules It has also been used as a mediator dye in cytochrome P450 spectral assay.

Safranin O has been used to stain matrix proteoglycans to study cartilaginous tissue structural integrity in mice. It has also been used to stain chondrogenically differentiated human bone marrow stromal cells.

Packaging

25, 100 g in glass bottle

Suitability

Certified as a stain for chromosomes in the Flemming triple stain; to stain gram negative bacteria in the Gram stain procedure; Safranine-fast green FCF stain for plant cells.

Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
SG1623000

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles
Peer-Reviewed Papers
15

References

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