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S9514 Sigma-Aldrich

Superose® 12 Prep Grade

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Properties

Related Categories Gel Filtration Chromatography, Gel Filtration Media, Molecular Biology, Protein Chromatography, Proteomics More...
form   aqueous ethanol suspension
particle size   20-40 μm (wet)
pore size   1,000-300,000 Da fractionation range (globular proteins)
storage temp.   2-8°C

Description

Application

Superose® 12 prep grade is used for protein chromatography, gel filtration chromatography and gel filtration media. Superose® 12 prep grade has been used to purify and characterize a haemolysin of Actinomyces pyogenes as well as a fibrinogenase from Vipera lebetina (desert adder) venom. Superose® 12 prep grade has also been used for the isolation and characterization of an extracellular protease of Actinomyces pyogenes.

Packaging

125 mL in glass bottle

Other Notes

Highly cross-linked beaded agarose

Physical form

Suspension in 20% ethanol

Legal Information

Superose is a registered trademark of GE Healthcare

Safety & Documentation

Safety Information

Symbol 
GHS02  GHS02
Signal word 
Warning
Hazard statements 
RIDADR 
NA 1993 / PGIII
WGK Germany 
3
Flash Point(F) 
100.4 - 109.4 °F
Flash Point(C) 
38 - 43 °C

Documents

Certificate of Analysis

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Protocols & Articles

Protocols

Performing an isolation of recombinant protein complexes

For structural studies and for comprehensive functional studies of multiprotein complexes it is often necessary to isolate protein amounts in the mg range. As mentioned above, pulldown methods are of...
Keywords: Chromatography, Dialysis, Gene expression, Ion Exchange, Scintillation, Tagged proteins

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

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