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S9626 Sigma-Aldrich

Sulfatase from Helix pomatia

Type H-1, sulfatase ≥10,000 units/g solid

Synonym: Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate and PTM Analysis,
Quality Level   200
type   Type H-1
form   powder
sulfatase activity   ≥10,000 units/g solid
secondary activity   ≥300 unit/mg solid β-glucuronidase (at pH 5.0)
mol wt   ~85 kDa by gel filtration
Featured Industry   Diagnostic Assay Manufacturing
shipped in   wet ice
storage temp.   −20°C

Description

Application

Sulfatase from Helix pomatia has been used in:
• sample extraction
• sulfatase assays
• enzymatic hydrolysis
• extract preparation for glucosinolate analysis

Packaging

5000, 10000, 100000 units in poly bottle

Biochem/physiol Actions

Sulfatase from Helix pomatia facilitates the synthesis of thiohydroximates. It causes the hydrolysis of β-naphthyl sulfate much faster than a-naphthyl sulfate and possesses regiospecificity in the hydrolysis of ortho and para substituted phenyl sulfates.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the solubility of Product S9626, Sulfatase from Helix pomatia?
Prior to determining the activity of this enzyme, Sigma reconstitutes it in 0.2% (w/v) sodium chloride.
Does Product S9626, Sufatase from Helix pomatia, contain B-Glucuronidase activity?
This product contains >300 units/mg solid B-Glucuronidase activity.
How is Product S9626, Sulfatase from helix pomatia, prepared?
This enzyme is isolated by acetone fractionation.
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Protocols & Articles

Protocols

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

This procedure may be used for determination of Sulfatase activity in products, except for Sulfatase products from Aerobacter aerogenes (Product Number S1629).
Keywords: Extinction coefficient

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Peer-Reviewed Papers
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References

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N3877 Potassium 4-nitrophenyl sulfate, sulfatase substrate

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