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S9751 Sigma-Aldrich

Sulfatase from Helix pomatia

Type H-2, aqueous solution, ≥2,000 units/mL

Synonym: Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate and PTM Analysis,
Quality Level   200
type   Type H-2
form   aqueous solution
secondary activity   ≥100,000 units/mL β-glucuronidase (at pH 5.0)
mol wt   ~85 kDa by gel filtration
concentration   ≥2,000 units/mL
shipped in   wet ice
storage temp.   2-8°C

Description

General description

Sequential action of sulfatase from Helix pomatia and β-O-glucosidase from Caldicellulosiruptor saccharolyticus on glucosinolates allows synthesis of thiohydroximates. Sulfatase from Helix pomatia hydrolyzes β-naphthyl sulfate much faster than α-naphthyl sulfate.

Application

Sulfatase from Helix pomatia is often used to remove sulfate groups from carbohydrates.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hour at pH 5.0 at 37 °C (30 min assay).

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Protocols

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

This procedure may be used for determination of Sulfatase activity in products, except for Sulfatase products from Aerobacter aerogenes (Product Number S1629).
Keywords: Extinction coefficient

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Peer-Reviewed Papers
15

References

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