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S9754 Sigma-Aldrich

Sulfatase from abalone entrails

Type VIII, lyophilized powder, 20-40 units/mg solid

Synonym: Aryl-sulfatase, Aryl-sulfate sulfohydrolase, Phenolsulfatase

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Properties

Related Categories 3.1.x.x Acting on esters, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate and PTM Analysis,
Quality Level   200
type   Type VIII
form   lyophilized powder
specific activity   20-40 units/mg solid
mol wt   ~43 kDa by gel filtration
foreign activity   β-Glucuronidase ≤10.0 Sigma units/mg solid
storage temp.   −20°C

Description

Application

Sulfatase from abalone entrails has been used in a study to determine that human p-selectin glycoprotein ligand-1 interacts with the skin-associated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus. Sulfatase from abalone entrails has also been used in a study to investigate HPLC of sulfate and glutathione conjugates from hamster embryo fibroblasts.

Sulfatase from abalone entrails has been used to digest the conjugated forms into free forms for the analysis of tissue green tea polyphenols (GTPs). It has also been used for the detection of free, glucuronidated or total form of GTPs.

Packaging

500, 1000, 5000 units in poly bottle

Biochem/physiol Actions

Sulfatases hydrolyze the sulfate esters of complex macromolecules, like glycosaminoglycans and sulfatides. It helps to modulate the sulfation states that determine the action of several physiological molecules.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C (30 min assay).

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Protocols

Enzymatic Assay of Sulfatase (EC 3.1.6.1.)

This procedure may be used for determination of Sulfatase activity in products, except for Sulfatase products from Aerobacter aerogenes (Product Number S1629).
Keywords: Extinction coefficient

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Peer-Reviewed Papers
15

References

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N3877 Potassium 4-nitrophenyl sulfate, sulfatase substrate

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