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S9809 Sigma-Aldrich

Anti-Sp1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for Gene Regulation, Antibodies to Common Transcription Factors,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   microarray: suitable
  western blot: 1:100 using a nuclear extract from HeLa human epithelioid carcinoma cells
species reactivity   human
mol wt   antigen 95-106 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   P08047
Gene Information   human ... SP1(6667)

Description

General description

Sp1 (Simian virus 40 protein-1) is a ubiquitous sequence specific nuclear transcription factor that recognizes GGGGCGGGGC and related sequences (GC boxes) on numerous viral and cellular genes. The sequence specificity of DNA binding is conferred by three c-terminal Cys2His2-type zinc -fingers and its affinity is regulated by a different region. Sp1 is an abundant phosphorylated and O-glycosylated protein that is expressed in most cells in levels dependent on cell type and developmental stage. It is a member of a family comprising Sp1, Sp2, Sp3, Sp4 and at least twelve additional Sp1 like members.

Immunogen

synthetic peptide of human Sp1, amino acids 436-454 with N-terminally added lysine, conjugated to KLH.

Application

Anti-Sp1 antibody produced in rabbit has been used in western blotting and immunoprecipitation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Sp1 (Simian virus 40 protein-1) has been implicated in maintenance of homeostasis and in cellular responsiveness to environmental changes. It mainly appears to be an activation transcription factor.

Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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