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S9915 Sigma-Aldrich

Stem Cell Factor from mouse

SCF, recombinant, expressed in E. coli, powder, cell culture tested

Synonym: SCF, c-Kit ligand

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Properties

Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors, Cytokines, Growth Factors and Chemokines for Stem Cell Differentiation,
biological source   mouse
recombinant   expressed in E. coli
assay   ≥95% (SDS-PAGE and HPLC)
form   powder
potency   ≤10 ng/mL ED50/EC50
quality   endotoxin tested
mol wt   18.3 kDa
packaging   pkg of 10 μg
  pkg of 50 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   <1 EU/μg
color   white
solubility   water: soluble
suitability   cell culture tested
UniProt accession no.   P20826
storage temp.   −20°C
Gene Information   mouse ... Kitl(17311), kitl(17311)

Description

Analysis Note

The biological activity is measured by the dose-dependent stimulation of the proliferation of human TF-1 cells.

Biochem/physiol Actions

Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells.

Stem cell factor is responsible for cellular development, proliferation, migration and survival, specially observed with cardiac stem cells. This phenomenon is mediated by stem cell factor (SCF) through chemotaxis. SCF is involved in repair mechanism of myocardial infarctions. SCF signalling is responsible for the function and survival rate of interstitial cells of Cajal. Decreased level of SCF leads to gastrointestinal motility disorders. SCF might be associated with systemic vascular remodeling. SCF signalling is also believed to contribute to the pathogenesis of pulmonary hypertension.

Physical form

Lyophilized from a 0.2 μm filtered buffered solution.

Application

SCF, also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.

Stem Cell Factor from mouse has been used in the evaluation of in vitro 5-lipoxygenase inhibition by measuring the formation of LTC4.

General description

Stem cell factor (SCF), also known as the c-kit ligand, is produced by the gastrointestinal smooth muscle cells, enteric neurons, endothelial cell, lung fibroblast and bone marrow adipocytes. The SCF gene is mapped to murine chromosome 10.
Recombinant mouse stem cell factor (SCF) is a 18.3 kDa protein containing 164 amino acid residues.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
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References

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