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SAB1300315 Sigma-Aldrich

Anti-ABCB5 (N-term) antibody produced in rabbit

saturated ammonium sulfate (SAS) precipitated, buffered aqueous solution

Synonym: Anti-ABCB5 P-gp, Anti-ABCB5, Anti-ATP-binding cassette sub-family B member 5, Anti-P-glycoprotein ABCB5

  •  NACRES NA.41

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Properties

Related Categories A1-AB, Alphabetical Index, Antibodies, Primary Antibodies
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   flow cytometry: suitable
  indirect ELISA: 1:1000
  western blot: 1:100-1:500
species reactivity   human
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   saturated ammonium sulfate (SAS) precipitated
Quality Level   100
antibody product type   primary antibodies
NCBI accession no.   NP_848654
UniProt accession no.   Q2M3G0
Gene Information   human ... ABCB5(340273)

Description

General description

ABCB5 (ATP binding cassette subfamily B member 5) gene is located in human chromosome 7p21.1. The encoded protein belongs to the subfamily B multidrug resistance and ATP-binding cassette superfamily of active transporters. The encoded protein have two identical halves, each contains a hydrophobic region with six transmembrane segments and a nucleotide-binding region. Expression of this gene is observed in prostate and testis and in human melanoma tumor-initiating cells.

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules.

Immunogen

ABCB5 (NP_848654, 3-35)
This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human ABCB5.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Flow cytometry/Cell sorting (1 paper)

Physical form

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

ABCB5 (ATP binding cassette subfamily B member 5) is a cell surface marker for melanoma-initiating cells, on cellular metabolism. ABCB5 significantly regulates glycolysis, glutaminolysis and phospholipid metabolism. It induces stem cell differentiation, stem cell immortality and anti-apoptotic activity. The gene is upregulated in many cancer subtypes, including breast cancer. Recent studies reveal that ABCB5 is a novel marker for limbal stem cell and is needed for corneal development and repair. ABCB5 shows resistance against taxanes and anthracyclines, the anti-cancer drugs.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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