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SAB1306481 Sigma-Aldrich

ANTI-CD45(C-TERMINAL) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: CD45, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200

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Properties

Related Categories Alphabetical Index, Antibodies, CDH-CDY, Primary Antibodies
clone   polyclonal
biological source   rabbit
application(s)   flow cytometry: 1:10-1:50
  immunohistochemistry: 1:10-1:50
  western blot: 1:1000
species reactivity   human
mol wt   mol wt 147254 Da
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
NCBI accession no.   NP_002829.3
UniProt accession no.   P08575
Gene Information   human ... PTPRC(5788)

Description

General description

Protein tyrosine phosphatase, receptor type C (PTPRC) or leucocyte common antigen CD45 is expressed on T-cells and is found in multiple isoforms. The extracellular domain of the protein is predicted to have an amino-terminal mucin-like segment, a cysteine-rich domain and three fibronectin type 3 domains. The gene encoding this protein is localized on human chromosome 1q31.3.

Application

ANTI-CD45(C-TERMINAL) antibody produced in rabbit has been used in Western blotting.

Physical form

Supplied in PBS with 0.09% (W/V) sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Protein tyrosine phosphatase, receptor type C (PTPRC) or leucocyte common antigen CD45 acts as a protein tyrosine phosphatase in leucocyte signaling. It has a role in flow cytometric diagnostics in leukemia. By dephosphorylation of Src kinases, the protein acts as a regulator of antigen receptor signaling in T and B cells. As a negative regulator of janus kinases, CD45 inhibits cytokine receptor signaling.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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